SILAC and Tandem Mass Tag Protein Analysis
We offer two powerful and analytical solutions to analyze the role of the proteome in cell development and to understand the mechanisms of stem cell differentiation.
Stable isotope labeling with amino acids in cell culture (SILAC) and Tandem Mass Tag (TMT) technology are able to identify and quantify proteins from complex samples such as those found in stem cell cultures.
The SILAC Method uses in vivo metabolic incorporation of "heavy" 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) analysis for accelerated comprehensive identification, characterization and quantitation of proteins.
Isobaric chemical tags are powerful tools that enable concurrent identification and quantitation of proteins in different samples using tandem mass spectrometry. They are small chemical molecules with identical structure that covalently attach to the free amino termini of lysine residues of peptides and proteins, thereby labeling various peptides in a given sample
Both methods are available as standalone sets or alternatively as optimized kits ready for the study of human mesenchymal or mouse embryonic stem cells.
Our complete family of isobaric tags called Tandem Mass Tags (TMT) are uniquely designed to enable a rapid and cost-effective transition from method development to high-throughput protein quantitation. The tags consist of TMT0 (zero), the TMT2 two-plex set and the TMT6 six-plex set. The TMT tags are provided as standalone sets or in optimized kit formats containing all necessary reagents and controls for maximum flexibility, convenience and reliability.
The SILAC kits are available optimized for human mesenchymal and mouse embryonic stem cell analysis. The kits contain optimized media for the stem cell propagation and SILAC protein expression profiling using mass spectrometry. The kits also include the high-quality stem cell ready screened and dialyzed FBS and several alternative isotopes of arginine and lysine that allow for the analysis of multiple growth conditions in each experiment.
The TMT and SILAC technologies combined with the industry-leading Thermo Scientific LTQ Orbitrap, LTQ XL and TSQ Quantum Mass Spectrometry Instrument platforms and software provide integrated total system solutions for quantitative protein expression analysis.
