High Content Reagent- and Cell-Based Assays


High Content	Instruments	Informatics	Assays	Publications

Make the next drug discovery breakthrough and study cell signaling pathways using Thermo Scientific* High Content Screening (HCS) and Analysis (HCA) assays. Whether your research is in cancer, toxicology, neurobiology, immunology or general cell biology, there’s an HCS reagent that can carry your research forward.

We offer both antibody- and dye-based reagents and EGFP (Enhanced Green Fluorescent Protein) reporter cell lines for Redistribution Assays. Combine the antibody- and dye-based reagents with the EGFP reporter cell lines to assay multiple cellular targets, saving time and reagent costs. For the "Total Solution", use high-content reagents with our HCS instrumentation and informatics for automated, high-throughput, cell-based, quantitative imaging and analysis of molecular targets.

Highlights:

Flexible - discover drug targets, study cell signaling pathways and perform immunofluorescence experiments
High quality - highest possible sensitivity, specificity and signal-to-noise ratios
Customizable - antibody- and dye-based reagents and EGFP reporter cell lines are offered separately allowing you to customize experiments to your needs and budget
Multiplex capabilities - combine antibody- and dye-based reagents with EGFP reporter cell lines for multiple readouts from the same cell in a single assay run
High-throughput - for large-scale studies, use our instrumentation and software designed for automated high content screening

Reagent-Based Assays

HCS Antibody and Dye-Based Reagents Used for High Content Microscopy

Antibodies, fluorescent probes, and reagents compatible for HCS and conventional fluorescence microscopy

Many reagents are now sold individually instead of as kits to provide flexibility in studying multiple targets.

Download a PDF listing of all reagent-based assays available to order.

Assays: || Antibody | Dye | Chemotaxis || Buffers

Antibody-Based Assays

Cell Cycle and Cell Proliferation

BrdU (Cell Proliferation)
BrdU and Ki67 (Cell Proliferation)
BrdU and Phospho-Histone H3
Cell Cycle I (BrdU, Phospho-Histone H3)
Cell Proliferation (BrdU, Ki67)
Cyclin B1 Activation
Ki67 (Cell Proliferation)
Mitotic Index (Phospho-Histone H3)
Phospho-PLK1 Activation
Phospho-mTOR Activation
Phospho-Rb Activation

Cell Morphology and Phenotypic Change

Cytoskeletal Rearrangement (F-actin and Whole Cell Probes)
Cytoskeletal Rearrangement (F-actin, Tubulin and Whole Cell Probes)
Cytoskeleton (Tubulin and Whole Cell Probes)
F-actin (Cytoskeletal Rearrangement)
Neurite Outgrowth
Nuclear Morphology (Cytoskeletal Rearrangement)
Synaptogenesis

Cell Signaling and Transcription Factor

ATF-2
Beta-Catenin
ERK MAPK
FOXO1A (Transcription Factors)
FOXO3A
FOXO3A, Hif1alpha and Phospho-CREB
HIF-1alpha (Transcription Factors)
Phospho-AKT
Phospho-CREB (Transcription Factors)
Phospho-GSK-3
Phospho-JNK
PKA
PKA and Phospho-CREB
PKCalpha
Smad3 (Transcription Factors)
Smad3 and Phospho-CREB
Smad4

Cytotoxicity and Apoptosis

Autophagy (LC3B)
Autophagy (LC3B and Poly-Ubiquitin)
Caspase 3
Caspase 9
Cell Death (Multiparameter)
Cleaved PARP
Cytochrome C
Cytotoxicity 3 (Multiparameter)
Mitosis and Apoptosis
LC3B and Poly-Ubiquitin (Autophagy)
Poly-Ubiquitin Detection
Proteasome Function (Ubiquitin and LC3B)

Genotoxicity and DNA Damage and Repair

Ku70/80
MDM2 and p53
MDM2
p21 Activation
p53 Activation
p53 and p21
Phospho-ATM
Phospho-ATM and p53
Phospho-Chk2
Phospho-H2AX
Phospho-p53
Phospho-p53 and p53

Inflammation and Cell Stress

CHOP/GADD153
COX-2 Activation
Heme Oxygenase 1
Heme Oxygenase 1 and Phospho-p38
Hsp27 and Phospho-Hsp27
Hsp27 Detection
Hsp60 and Hsp90beta
Hsp60 Detection
Hsp70 and Hsp90alpha
Hsp70 Detection
Hsp90apha Detection
Immunophilin FKBP52
iNOS Activation
MnSOD and Phospho-H2AX
MnSOD
NFAT-1
NFkappaB
NFkappaB and c-Jun
p38 MAPK
Phospho-4E-BP1
Phospho-c-Jun
Phospho-c-Jun and Phospho-JNK
Phospho-Hsp27
Phospho-S6
STAT1, STAT2 or STAT3

Chemotaxis and Cell Motility Assays

Buffers and Accessory Reagents

Redistribution Cell Lines

Stable cell lines expressing the molecular target of interest fused to EGFP compatible for HCS and conventional fluorescence microscopy

EGFP Reporter Cell Lines

Cell Signaling and Oncology

G Protein Signaling

Inflammation

Intracellular Trafficking

Metabolic Disease and Nuclear Hormone Receptors

Neurology and Neuroscience

Neuroscreen-1

Protein Kinases, Protein Degradation, and Protein-Protein Interactions