HPLC and UHPLC for Biologics Development, Identification & QC – Now You Just Need One System!

Drug development workflows in biopharmaceutical laboratories can be demanding, requiring extensive data, accurate results, and fast separations. The Thermo Scientific* Dionex* UltiMate* 3000 Biocompatible Rapid Separation (BioRS) system perfectly matches the demands for biomolecule separations to run various modes of chromatography in UHPLC. This goes beyond reversed-phase UHPLC: it is genuine Bio UHPLC for proteins, peptides, and biotherapeutics.

Selection of main features:

• Pressure of up to 100 MPa (15,000 psi)
• Flow rates of up to 8 mL/min
• Short sampler cycle times (< 20 seconds)
• Wide column thermostatting range (5 °C to 110 °C)
• Ultrafast data collection and processing (up to 200 Hz)

The biocompatible UltiMate 3000 BioRS system was featured at the WCBP meeting in Washington, DC. Workflow solutions were presented on the latest technology and applications for protein therapeutics.

Webinar:  "New Developments in Chromatography for Improved Biopharmaceutical Analytics" New solutions to support characterization, comparability, similarity, and stability studies required during biotherapeutic and biosimilar drug development and QA/QC. Register here.

Webinar:   "HIgh Speed, High Resolution Characterization of Biomolecules" Using smaller particles for high resolution and high speed variant analysis. Register here. 

Rely on BioUHPLC Every Time, Everywhere.

Ultimate Productivity and Flexibility

Unique Column Chemistries

Seamless Integration with Mass Spectrometry

The system was developed for reliability and robustness under the harsh solvent conditions applied in biochromatographic analyses. Corrosion-resistant materials in the complete system flow path reduce the risk of analyte interaction with internal surfaces and extend the lifetime of particular biocolumns, even under high salt or extreme pH conditions.

The UltiMate 3000 BioRS system is a modular platform that offers several options for each component. All are fully biocompatible and UHPLC capable. This means that the UltiMate 3000 BioRS can be configured for any bio UHPLC analysis to deliver optimal separations at ultrahigh speed while maintaining resolution.

The gold standard Thermo Scientific* ProPac* WCX and MAbPac* columns can be used with salt or pH gradient LC. In addition new chemistries like the Thermo Scientific* GlycanPac* AXH-1 column are available to separate both labeled and unlabeled N- and O-glycans by charge, size, and isomer.

The UltiMate 3000 BioRS system combines easily with our best-in-class mass spectrometers. Routine confirmation on iontrap or triple quad mass spectrometers, or discovery on high resolution Thermo Scientific* Q Exactive*, Exactive Plus* or Thermo Scientific* Orbitrap* hybrids, all seamlessly integrate with the UHPLC+ solutions offered by the UltiMate 3000 BioRS system.