SkanIt Software Protocols for the Thermo Scientific Multiskan GO

This protocol library includes ready-made software protocols that can be used to run commercially available or commonly well-known assays with the Thermo Scientific Multiskan GO Microplate Spectrophotometer. The files contain protocols for the Thermo Scientific SkanIt Software, which is the PC software used to control the instrument and to perform calculations to measurement data. The instrument can also be used without a PC, but these protocols are compatible only with SkanIt Software.

The protocols can be downloaded from this website and imported to the database of the SkanIt Software for Multiskan GO. The software protocols are instrument-specific, therefore, they cannot be imported to the software of any other instrument. The protocol files cannot be viewed outside the SkanIt Software.

All the protocols are made for 96-well microplate format.

The protocols have been made according to the available kit instructions. The user performing the assay has the ultimate responsibility to verify that the definitions, parameters and calculations of the protocol match the kit instructions and the assay requirements. Thermo Fisher Scientific will not assume any responsibility for the results of the assays performed with these software protocols.

Read first the instructions on downloading the SkanIt Software protocols of the Multiskan GO.
Assay	Description	SkanIt Software Protocol Download
dsDNA quantitation	dsDNA quantitation and 260/280 ratio calculation
Promega CellTiter 96* AQueous One Solution Cell Proliferation Assay	Determining the number of viable cells in proliferation or cytotoxicity assays
Lonza LAL Kinetic-QCL Endotoxin	Quantitative detection of Gram negative bacterial endotoxin
Oxford Biochemical Research Total Antioxidant Power	Measurement of total antioxidant capacity of biological specimens
Thermo Scientific Coomassie (Bradford) Protein Assay	Total protein quantitation
Thermo Scientific Coomassie Plus (Bradford) Protein Assay	Total protein quantitation
Thermo Scientific 660 nm Protein Assay	Total protein quantitation
Thermo Scientific BCA Protein Assay	Total protein quantitation
Thermo Scientific Modified Lowry Protein Assay	Total protein quantitation
Thermo Scientific Human TNF Alpha ELISA	Quantification of human Tumor Necrosis Factor alpha
Thermo Scientific Human IFN gamma ELISA	Quantification of human Interferon gamma
Thermo Scientific Mouse IFN gamma ELISA	Quantification of mouse Interferon gamma
Thermo Scientific Human IL-8 ELISA	Quantification of human Interleukin-8
Thermo Scientific Human IL-1 beta ELISA	Quantification of human Interleukin-1 beta
Thermo Scientific Human IL-2 ELISA	Quantification of human Interleukin-2
Thermo Scientific Human IL-6 ELISA	Quantification of human Interleukin-6
Thermo Scientific Mouse TNF alpha ELISA	Quantification of mouse Tumor Necrosis Factor alpha