Solid supported liquid/liquid Extraction (SLE) is a fast, effective sample preparation technique that provides considerable benefits over liquid-liquid extraction (LLE) protocols for removal of phospholipids from biological samples. HyperSep* SLE offers the following advantages:
Greater reproducibility and recoveries compared to LLE techniques.
Prevents emulsification often associated with LLE as the sample and water immiscible solvents are not in direct contact.
Reduced solvent requirements compared to LLE
Can be completely automated unlike LLE
Improved cleanliness of sample extract compared to protein precipitation techniques
Improved sensitivity compared to protein precipitation techniques
HyperSep SLE is available in cartridge and 96-well formats to suit sample size and throughput requirements. The stationary phase consists of specially treated diatomaceous earth and is available in acidified or basified formats to help simplify the protocol when acidic or basic mobile phases are used.
Pipette aqueous sample into cartridge. (Add Internal Standard prior to loading. Sample may need diluting to aid adsorption or controlled with pH to suppress ionization (for bases add 0.1% Ammonia solution(aq). For Acids add 0.1% Formic Acid(aq)) maximum load including dilution solution = ~650µl).
Apply a pulse of vacuum to start loading. Wait 5 minutes.
Ensure top frit is dry by applying a pulse of vacuum.
Elute with a water immiscible solvent (e.g. MTBE).
Allow to flow through under gravity, using vacuum at the end to dry.
Evaporate solvent and reconstitute sample in mobile phase.
Available For Download
Register now for access to the following literature:
Application Note: The Extraction of Banned Azo Dyes in Textiles Using HyperSep SLE
Application Note: The Extraction Rifabutin from Serum Using HyperSep SLE
Application Note: The Extraction of Oxymorphone and its Isomer from Human Plasma Using HyperSep SLE
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