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Diagnostics




Our diagnostic solutions benefits from more than 30 years of industry-leading experience in immunoassay plate technology. Find valuable information abour products relating to passive binding surfaces for biomacromolecules, covalent coupling surfaces for smaller biomolecules, and affinity capture surface for affinity-tagged biomolecules in this diagnostic solutions section.

Choose your surface 


  • Diagnostic surfaces »

    Surfaces for ELISA
    The choice of the optimal surface for the binding or adsorption of the first layer in a sandwich ELISA is critical for the success of the procedure. While high binding is clearly important, the most important parameter is the detection limit which depends on the signal to noise ratio. Use the Thermo Scientific* Nunc* triangle (for passive adsorption surfaces) or the hexagon (for active binding) as a guide when selecting the surface for a new assay. To achieve the best possible results always optimize the incubation conditions and concentrations of the assay components.

    Thermo Scientific MaxiSorp*
    Nunc MaxiSorp is treated to give a mixture of hydrophobic and hydrophilic areas for the adsorption of proteins. It is particularly optimized for the adsorption of IgG in a sandwich ELISA.

    Thermo Scientific MediSorp*
    When high ELISA background readings are a problem, MediSorp may be the solution. MediSorp’s lower total adsorption capacity can, in many cases, reduce non-specific adsorption of serum proteins giving an improved signal to noise ratio.

    PolySorp*
    The PolySorp surface is ideal for the adsorption of hydrophobic molecules, including those that contain lipid moieties.

    Thermo Scientific MultiSorp*
    This highly hydrophilic surface has a number of applications. Use MultiSorp for the adsorption of hydrophilic biomolecules such as highly substituted glycoproteins. Alternatively this surface could be used for the assay, in liquid phase of very hydrophobic analytes.

    Streptavidin
    The interaction between biotin and streptavidin has been exploited in many assay applications. Nunc provides two types of streptavidin surface.

    Streptavidin Passive Attachment
    The streptavidin molecule can be adsorbed directly to the polystyrene surface. For many applications this works well, particularly for relatively small biotinylated molecules where steric hindrance due to closeness to the polystyrene presents no problem.

    Immobilizer* Streptavidin
    In this case the streptavidin molecules are covalently bound to the surface via spacer approx. 2 nm in length. This produces a number of advantages: reduced steric hindrance, a very stable surface and controlled spacing of the available binding sites, which is ideal for large biotinylated biomolecules.

    Immobilizer Amino
    In an ELISA, there are many advantages of covalently binding the first layer to the surface. The Amino surface can be used to covalently bind proteins (including antibodies) or peptides. With a modifi ed protocol DNA can also be bound. Once the first layer is bound it is extremely stable allowing rigorous washing in the subsequent steps.

    Immobilizer Nickel-Chelate/ Immobilizer Glutathione
    For determination and characterization of fusion proteins, Nunc Brand offers two possibilities. Nickel-Chelate for Histidine-tagged and Glutathione for GST-tagged fusion proteins. These plates can be used for purified samples, as well as crude cell lysates. Both Immobilizer Nickel-Chelate and Glutathione have all the advantages of the Immobilizer family of products: no activation steps, low detection limit, high signal to noise ratio and low background without blocking.

    Thermo Scientific CovaLink*
    The CovaLink surface chemistry often allows binding of molecules, where other methods have failed. It can be used with bifunctional linkers to bind peptides via a carboxylic acid group or DNA by the phosphorylated 5' end of the molecule DNA. Alternatively, the whole surface can be activated using glutaraldehyde.

    Thermo Scientific Immulon* 1 B
    Consistent medium binding for hydrophobic complexes and proteins.

    Immulon 2 B
    High binding surface to provide increased binding of hydrophilic proteins and complexes.

    Immulon 4 HBX
    Extra-high binding surface with special resin - offers maximum protein uptake Well-to-well CV’s of plates is certified.

    Microfluor 1 White
    Medium binding - offers high signal reflectance and reduced background fluorescence.

    Microfluor 2 White
    Microplate for assays that require high binding and reduced background for fluorescence assays in the ultraviolet range.

    Microfluor 1 Black
    Fluorescence vessel with low background

    Microfluor 2 Black
    Irradiated Microtiter microplate that provides higher binding and minimal background fluorescence.

    Microlite 1+
    Ideal for low signal luminescent reactions, offering medium binding with enhanced dynamic range, extra high reflectivity and minimal cross talk.

    Microlite 2+
    High binding surface with enhanced dynamic range, extra high reflectivity and minimal cross talk for low signal luminescent reactions.

  • Surfaces for passive binding »

    Schematic representation of the types of biomacromolecules, which can be bound to the available modified surfaces. E.g., if a lipid is to be bound, the hydrophobic surface Nunc PolySorp is most suitable. Based on the physiochemical characteristics of the biomolecule to be immobilized, a surface can be chosen which is appropriate for robust binding. As is indicated in Fig. 1, Nunc MaxiSorp has the widest breadth applications, as it is capable of binding the greatest range of molecules.

    Figure 1 - Surfaces for passive binding

    Surface characteristics:

    • Absorb larger biomolecules
    • Different molecular orientations are possible
    • A variety of surfaces are available for performance optimization

    Table 2. Passive binding surfaces characteristics

    Click table to enlarge

    * Release tested for binding reproducibility.

    Abbreviations:

    • FIA - Fluorescent Immunoassay
    • LIA - Luminescent Immunoassay
    • NA - Nucleic Acid
    • ELISA - Enzyme Linked Immuno Sorbent Assay
    • PCR - Polymerase Chain Reaction
    • LPS - Lipopolysaccharide
    • EDC - 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide
    • CV - Correlation of variation

    For additional information, visit our tech notes/application notes library.

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    Surfaces for passive absorption

    Whatever the format chosen, there are a number of choices to be made about the best type of surface for the application. The choice will depend on the assay component to be immobilized.

    Passive surfaces have a broad range of applications, as they can bind a variety of biomolecules, based on multiple weak molecular interactions forming a stable bond. Passive binding is therefore primarily suited for the immobilization of medium- to large-sized molecules, such as antibodies, which are capable of establishing several contact points. The exact molecular interaction sites are dependent on the specific matching of the biomolecule’s properties with the polymer surface. A large variety of biomolecules can be stably immobilized on passive surfaces with good residual activity.

    The family of Thermo Scientific passive surfaces vary in their degree of hydrophilicity and are organized into four different sub-groups: hydrophobic, slightly hydrophilic, hydrophilic, and very hydrophilic. A hydrophobic surface functions predominantly via hydrophobic binding. With the increased hydrophilicity caused by the incorporation of oxygen containing functional groups, electrostatic interactions can play a greater role in binding, thus influencing which types of biomolecules will bind strongly to the surface.

    Hydrophobic

    • These surfaces are typically used for the adsorption of hydrophobic molecules such as lipid rich biomolecules.

    Slightly hydrophilic

    • The slight hydrophilicity of these plates enhances their ability to bind a diverse range of biomolecules, including glycoproteins, serum containing samples and amphoteric molecules such as lipopolysaccarides. With these plates non-specific adsorption of serum containing samples are reduced, and this will improve the signal-to-noise (S/N) ratio and consequently, sensitivity levels.

    Hydrophilic

    • Optimized to bind high amounts of IgG (polyclonal), these plates are ideal for antibody sandwich assays (e.g. ELISAs). In addition, they show increased binding of many other proteins and biomolecules that possess hydrophilic/hydrophobic characteristics.

    Very hydrophilic

    • The most hydrophilic in our portfolio will bind many hydrophilic proteins with a high affinity to these plates. Binding does, however, tend to be more pH sensitive.

    Table 1, Table 2 and Fig. 1 show the link between the surface and the affinity of the biomolecule.

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    Table 1. General guidelines for the selection of passive surfaces for the immobilization of particular biomolecules.

    BIOMOLECULE: The likelihood of effectively immobilizing a biomolecule on a particular surface is indicated in the table

    Click table to enlarge

  • Surfaces for covalent binding »

    Covalent coupling, is based on the formation of a single covalent bond between the polymer surface and the biomolecule. Small biomolecules can be immobilized using this technique, as can medium and large molecules that possess the appropriate functional group(s). Since coupling occurs via specific functional groups, biomolecular orientation can also be manipulated by the user.

    Figure 2 - Surfaces for covalent binding

    Surface characteristics:

    • Strong covalent bonds firmly anchor biomolecules
    • Compatible with vigorous washing
    • Stability is provided
    • Coating with lower amounts of reagent may be possible
    • Control of molecular orientation

    Figure 3 - Surfaces for covalent binding

    Thermo Scientific Nunc CovaLink

    • The CovaLink* surface is designed for coupling of molecules bearing a free carboxyl or phosphate group. Therefore, peptides, haptens and DNA can be coupled. The surface uses a spacer arm to increase accessibly, thereby enhancing overall surface reactivity (Fig. 2).

    Thermo Scientific Nunc Immobilizer Amino

    • The Immobilizer* Amino surface forms stable covalent bonds between its electrophilic groups and the biomolecule’s free amino acids or sulfhydryl groups. Using its unique spacer arm chemistry, the surface provides extremely low non-specific binding to improve assay sensitivity. With no need for an ancillary coupling agent, this surface can simplify your assay development by eliminating the need for a time-consuming blocking step (Fig. 3).

    The Immobilizer Amino surface is ideal

    • If your biomolecule does not bind well to a passive surface, and it possesses one or more free primary amino or sulfhydryl groups (peptides, oligonucleotides, proteins, proteoglycans)
    • To obtain a highly sensitive assay with excellent reproducibility and low background
    • To minimize the use of a coating reagent
    • To reduce the number of steps required to prepare plates
    • To avoid unwanted reactivity associated with a blocking reagent

    To help you find the best products for your applications, visit our online plate selection tool at: www.thermoscientific.com/plateguide

    Covalent coupling surfaces

    • The diagram shows the available surface modifications for directed binding of target biomolecules. In the case of Immobilizer Amino and CovaLink, a strong covalent bond is formed with the biomolecule being immobilized at the surface. The biomolecule must possess the correct functional group for covalent coupling. The relevant functional groups on the biomolecule are shown in Fig. 4.

    Figure 4 - Surfaces for covalent coupling

    Table 3 shows the link between the surface and the binding preference.

    Table 3. Covalent Coupling Surfaces

    Click table to enlarge

    * Release tested for binding reproducibility.

    Abbreviations:

    • FIA - Fluorescent Immunoassay
    • LIA - Luminescent Immunoassay
    • NA - Nucleic Acid
    • ELISA - Enzyme Linked Immuno Sorbent Assay
    • PCR - Polymerase Chain Reaction
    • LPS - Lipopolysaccharide
    • EDC - 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide
    • CV - Correlation of variation

    For additional information, visit our tech notes/application notes library.

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  • Affinity capture surfaces »

    Affinity capture is based on the specific binding of a tagged biomolecule to its receptor. The plate surface is therefore developed with one of the binding pair (the receptor) immobilized on its surface, while the tag is linked to a biomolecule either by chemical coupling or by genetic engineering. The tagged biomolecule can then be captured on the plate surface with a high degree of specificity.

    Figure 5 - Affinity capture surfaces

    Surface characteristics:

    • Affinity capture surfaces have highly specific binding
    • Reduced variability in molecular orientation
    • Immobilizer surfaces improve S/N ratios
    • Streptavidin biotin interaction can be exploited to immobilize a wide range of biomolecules (proteins, peptides, haptens, nucleic acids)

    Passive Streptavidin

    Streptavidin is passively coated on the plate with a biotin binding capacity of at least 13-20 pmol per well.

    Immobilizer Streptavidin

    The streptavidin protein molecules are covalently bound to the surface via a spacer arm to reduce leaching and enhance precision. The surface is also modified to minimize non-specific binding. As a result, a high S/N ratio is produced, for increased sensitivity. A biotin binding capacity of 20 pmol per well produces excellent analytical results (Fig. 5).

    Figure 6 - Affinity capture surfaces

    Immobilizer Nickel Chelate

    A nickel chelate group is attached to the polymer surface via a spacer arm and will bind polyhistidine, which is typical genetically engineered into a fusion protein. The spacer arm design maximizes the reactivity of the surface, while minimizing non-specific binding, and covalent linkage significantly reduces leaching. The surface does not need to be blocked and therefore produces a high S/N ratio (Fig. 6).

    Figure 7 - Affinity capture surfaces

    Immobilizer Glutathione

    A GST peptide, attached to the polymer surface via a spacer arm, will bind glutathione, which is typical genetically engineered into a fusion protein. The spacer arm maximizes surface reactivity while minimizing non-specific binding, and the covalent linkage reduces the occurrence of leaching. The surface does not need to be blocked and therefore a high S/N ratio is obtained (Fig. 7).

    The diagram shows the available surface modifications for directed binding of target biomolecules.

    Table 4 shows the link between the surface and the binding preference.

    Figure 8 - Affinity capture surfaces

    Click table to enlarge

    *Release tested for binding reproducibility.

    Abbreviations:

    • FIA - Fluorescent Immunoassay
    • LIA - Luminescent Immunoassay
    • NA - Nucleic Acid
    • ELISA - Enzyme Linked Immuno Sorbent Assay
    • PCR - Polymerase Chain Reaction
    • LPS - Lipopolysaccharide
    • EDC - 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide
    • CV - Correlation of variation

    For additional information, visit our tech notes/application notes library.

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Custom Capabilities 

Thermo Scientific offerings include a range of customized options to meet your specific application requirements.

  • Color Coding »

    We may offer to supply Nunc modules as color coded modules upon request and with a minimum order size. Standard colors are available. For details, please contact your local Nunc brand distributor, or e-mail info.nunc@thermofisher.com.

    • Standardized to facilitate easy identification of your assays
    • Makes it possible to use different colors for patient identification in one frame
    • High reliability when working with individual wells
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  • Barcoding »

    For customized products (with a minimum annual quantity), we offer a choice of barcode options. A barcode provides a safe way to keep track of samples and helps reduce human errors. For details, please visit www.barcodeconfigurator.com and contact your local distributor.

    • Improved accuracy
    • Increased efficiency
    • Higher safety levels
    • Reduced costs
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  • Custom Coating »

    We offer an extensive knowledge of surface treatment, capabilities and production skills. We can custom coat plates and modules based on custom-specific protocols. Add your reagents, and we provide the final coated plates with consistent, reproducible quallity, along with requested documentation. This provides you with the optimal flexibility in your production line. For more information, contact your local Nunc brand distributor or e-mail us at info.nunc@thermofisher.com.

    • Frees up resources, saves cost and reduces investment
    • Leverage proven strengths in surface treatment, surface capability and production

Custom Products for In Vitro Diagnostics 

Your proven strategic partner for development and manufacturing in the IVD industry

  • Our commitment to the diagnostic industry »

    Known worldwide for superior quality and innovative products, you can depend on Thermo Fisher Scientific* to design, develop and manufacture customized solutions tailored to your exacting requirements.

    As leaders in leakproof containment systems, diagnostic surfaces and plastic materials, we combine world class manufacturing with industry-leading development practices to deliver innovative system solutions with on-time implementation and delivery.

    As your partner, we provide:

    • Extensive R&D capabilities
    • Global footprint for localized manufacturing
    • Product and process validation expertise
    • Wide range of development and manufacturing technologies for plastic products
    • Supply chain management proficiency
    • Quality systems designed to provide support in meeting global regulatory standards
    Get the Brochure

    Thermo Scientific Nalgene* diagnostic leakproof bottles and Nunc diagnostic products set the standard for quality and innovation - and by continuing to deliver leading solutions, we have become one of the life science industry’s most trusted partner.

    Our design and development experience enables you to deliver innovative solutions to your customer.
    We are a high value development and production partner for all aspects of plastic custom products.

    Contact Information:

    Email: customconsumables@thermoscientific.com

  • Reagent packaging & diagnostic devices »

    Optimize your opportunities

    • Leakproof container and closure systems
    • Validated and certified materials
    • ISO 13485 quality system
    • World class diagnostic components
    • Expert guidance on surface selection
    • Tailored for your application
    • Exact solutions
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  • Capabilities »

    Design

    • Industrial and human factors
    • Mechanical engineering
    • Design for manufacturability
    • Proven IVD product experience

    Development

    • Project management
    • Prototype and pilot scale tools for design verification
    • Plastic materials expertise to help drive innovation solutions for molded products

    Manufacturing

    • Global manufacturing locations to optimize your supply chain
    • Cleanroom molding capabilities
    • Broad plastic processing capabilities
    • Automated assembly processes
    • Surface modification/treatments

    Validation

    • Quality process tools throughout project development
    • PFMEA and risk assessment processes
    • Complete validation documentation support

    Regulatory support

    • Materials compliance to local regulations
    • Support documentation for your process analysis

    General capabilities

    • Diagnostic assay and reagent services that can be combined with our plastic custom consumable development processes
    • Immunoassays
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Diagnostic Product Features 

Immuno Plates Clear
Immuno Plates Clear

Immuno Breakable Modules Clear
Immuno Breakable Modules Clear

Immuno Standard Modules Clear
Immuno Standard Modules Clear

Immuno Plates White and Black
Immuno Plates White
and Black

Immuno Breakable Modules White and Black
Immuno Breakable Modules White
and Black

Immuno Standard Modules White and Black
Immuno Standard Modules White and Black

Immuno Specialty Surfaces
Immuno Specialty Surfaces

Immuno Tubes and Stoppers
Immuno Tubes and Stoppers

Diagnostic Bottles: Nalgene small volume bottles 4-15ml
Diagnostic Bottles: Nalgene small volume bottles 4-15ml

 
 
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