Blocking Buffers for Protein Methods
Traditional protein blocking agents (BSA, casein and milk) and exclusive Pierce* Protein Research blocking buffers (SuperBlock*, StartingBlock*, Protein-Free) for Western blotting, ELISA and other immunoassay detection methods with microplates and membranes.
Complete, non-interfering blocking of nonspecific binding sites is increasingly critical and difficult to achieve with more sensitive protein detection methods, such as chemiluminescence. We've developed some of the most sensitive immunodetection products available; you can depend on us to offer optimized and high-performance blocking reagents to match.
Western blot and ELISA Blocking Buffer Selection Guide »
Blocking Strategies for Immunohistochemistry (IHC) »
Thermo Scientific* Blocker BLOTTO Blocking Buffer is a ready-to-use 5% solution of nonfat powdered milk in Tris-buffered saline for using in Western blotting, ELISA, immunohistochemistry and other detection methods.
Thermo Scientific* Blocker BSA Blocking Buffers are ready-to-use, 10X PBS or TBS solutions of bovine serum albumin protein for blocking steps in Western blotting, ELISA, immunohistochemistry and nucleic acid detection methods.
Thermo Scientific* Blocker Casein Blocking Buffers are ready-to-use, PBS or TBS solutions of purified casein protein for blocking steps in Western blotting, ELISA, immunohistochemistry and nucleic acid detection methods.
The Thermo Scientific* Pierce Clear Milk Blocking Buffer (10X) is a long shelf-life, high performance replacement for homemade milk blocking buffers in Western blotting applications.
Thermo Scientific* Pierce Fast Blocking Buffer is a ready-to-use blocker solution for optimizing Western blotting systems by decreasing both background signal and incubation time compared to traditional blocking agents.
Thermo Scientific* Pierce Normal Sera are non-immune serum samples from goat, human, mouse, rabbit and other species that are popular blocking agents for immunohistochemical (IHC) staining and cellular imaging methods.
Thermo Scientific* Pierce Protein-Free Blocking Buffers are PBS and TBS formulations of a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low backgrou
Thermo Scientific* SEA BLOCK Blocking Buffer is steelhead salmon serum (fish serum) in phosphate-buffered saline (PBS) that is especially useful as a blocking agent in immunohistochemistry (IHC) and other detection methods involving mammalia
Thermo Scientific* StartingBlock Blocking Buffers are PBS and TBS formulations of a proprietary protein blocker that provides exceptional nonspecific blocking and broad compatibility with membrane (Western blotting) and 96-well plate (ELISA)
Thermo Scientific* SuperBlock Blocking Buffers are optimized PBS and TBS solutions containing a protein that provides incredibly fast and effective blocking for ELISA, immunohistochemistry and Western blotting applications.