Modification Reagents for Amino Acids
Chemical agents to modify amino acid side chains on proteins and peptides in order to alter native charges, block or expose reactive binding sites, inactivate functions, or change functional groups to create targets for crosslinking and labeling.
There's no need to confuse and overwhelm yourself by attempting to search in a standard chemical catalog to find reagents for altering amino acid groups in proteins. We've organized a product grouping that includes just the most popular and versatile compounds for this purpose.
Crosslinkers at a Glance »
Crosslinker Selection Guide »
Crosslinking and Protein Modification Methods »
The Thermo Scientific* GlycoLink Coupling Catalyst decreases reaction times and increases aldehyde-hydrazide coupling efficiency, resulting in greater than 90% coupling of glycoproteins in 4 hours.
Thermo Scientific* Pierce N-Ethylmaleimide (NEM) is a small compound that forms stable, covalent thioether bonds with sulfhydryls (e.g., reduced cysteines), enabling them to be permanently blocked to prevent disulfide bond formation.
Thermo Scientific* Pierce AEDP is an amine-and-carboxyl (amino acid) compound that contains an internal disulfide bond, features which enable construction, extension and modification of several functional groups on proteins and surfaces.
Thermo Scientific* Pierce Citraconic Anhydride reacts with lysines and other primary amines to stably cap them with a carboxyl group at pH 7, but whose linkage can be completely reversed (hydrolyzed) using acidic conditions (pH 3 to 4).
Thermo Scientific* Pierce EMCA is a carboxylic acid compound with a terminal maleimide group that can be used to modify proteins and other molecules by EDC-mediated and sulfhydryl-targeted conjugation.
Thermo Scientific* Pierce Ethylenediamine Dihydrochloride is a simple compound that can be used with EDC (carbodiimide) crosslinker to polymerize or amine-modify carboxylate molecules for a variety of applications.
Thermo Scientific* Pierce Hydroxylamine-HCl exposes sulfhydryls of SATA-modified proteins and cleaves Asn-Gly bonds.
Thermo Scientific* Pierce Iodoacetic Acid (IAA) has many uses, including to provide S-carboxymethylation of sulfhydryls (reduced cysteines) at slightly alkaline pH.
Thermo Scientific* Pierce MMTS is methyl methanethiosulfonate, a small compound that enables cysteines and other sulfhydryl groups to be reversibly blocked to facilitate study of enzyme activation and other protein functions.
Thermo Scientific* Pierce SAT(PEG)4 is a long-arm (18.2 Ås), pegylated form of SATA, a sulfhydryl-addition reagent for covalent modification of primary amines to facilitate controlled, heterobifunctional crosslinking.