Western Blotting, ELISA and Cell Imaging
Blocking buffers, secondary probe conjugates, and substrate detection reagents (colorimetric, chemiluminescent and fluorescent) for sensitive and consistent protein analysis by Western blotting, ELISA and immunohistochemistry (IHC).
For membrane-based or plate-based protein detection procedures, you can find the reagents and accessories that you need in our portfolio. Our wide selection of ready-to-use blocking buffers and, secondary antibodies, blotting membranes, pre-coated and custom-coated microplates, precipitating and soluble detection substrates, and other innovative products address nearly every blotting or ELISA assay development challenge you might encounter. You can rely on our strict quality standards to ensure that every Western blot or ELISA you perform with our reagents is successful.
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Western Blot Analysis »
ELISA Strategies »
Immunohistochemistry (IHC) »
Protein Detection Probes »
Antibody-Binding Proteins and Conjugates
Protein A, Protein G, Protein A/G, Protein L and other antibody-binding proteins available in purified form and as biotinylated or peroxidase-labeled (HRP) conjugates for secondary detection in microplate and membrane assays.
Immunohistochemistry Reagents
Tissue crosslinkers, cell fixatives, formaldehyde, permeabilization reagents, precipitating enzyme substrates, dyes and staining kits for immunohistochemistry (IHC) and other histochemical detection procedures.
Biotin-Binding Proteins and Conjugates
Streptavidin, NeutrAvidin and avidin protein conjugates for biotinylated antibody probe detection in ELISA and Western blot protocols; includes peroxidase and poly-HRP conjugates and high-intensity fluorescent and infrared conjugates.
Molecular Biology Reagents
Reagents for nucleic acid extraction, purification, electrophoresis and detection, including agarose, luciferin, Tris-EDTA buffers and phenol-chloroform solutions.
Blocking Buffers for Protein Methods
Traditional protein blocking agents (BSA, casein and milk) and exclusive Pierce* Protein Research blocking buffers (SuperBlock*, StartingBlock*, Protein-Free) for Western blotting, ELISA and other immunoassay detection methods with microplates and membranes.
Nucleic Acid Labeling and Detection
Reagents and kits for labeling, hybridization, electrophoresis and transfer of DNA, RNA and other nucleic acids for Southern blotting, Northern blot analysis, gel-shift assays (EMSA) and gene expression studies.
ELISA Reagents, Microplates and Accessories
Substrates, buffers, plates and various other supplies to develop and complete ELISA and EIA experiments; includes high-binding capacity coated microplates and colorimetric (TMB), chemiluminescent and fluorogenic enzyme substrates for HRP conjugates.
Western Blot Reagents, Kits and Equipment
Colorimetric, fluorescent and chemiluminescent Western blot substrates (Pierce ECL, SuperSignal); nitrocellulose and PVDF membranes; X-ray film and accessories; and antibody stripping buffers essential for immunoblotting detection procedures.
