Cell Lysis and Protein Extraction
Protease inhibitors, phosphatase inhibitors, detergents and whole cell lysis reagents designed for efficient organelle isolation, subcellular fractionation and protein solubilization from a variety of cell and tissue types.
Our Pierce* Cell Lysis Reagents, detergent solutions, fractionation kits and protease inhibitor cocktails provide unsurpassed quality and convenience for protein research applications. Choose from our wide selection of ready-to-use formulations that are optimized to produce high-yield cell lysates from bacteria, yeast, plants and mammalian cells and tissues. Proceed directly to purification or analysis with the assurance that you've recovered functional proteins and organelles in buffers that are compatible with the downstream procedure.
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Cell Lysis and Protein Extraction »
Cell Lysis Protein Extraction Reagents
Gentle whole cell lysis buffers for high-yield protein extraction and solubilization from E. coli and other bacteria, cultured mammalian cells and tissues, insect cells, yeast cultures, and flowering plants.
Organelle Isolation and Enrichment Kits
Optimized reagent kits for subcellular fractionation of proteins and isolation of organelles such as nuclei, mitochondria, peroxisomes and lysosomes by Dounce-homogenization or density-gradient separation.
Detergent Removal Resins and Kits
Affinity columns and precipitation reagents to remove detergents and surfactant micelles from protein extracts and other processed biological samples; eliminate detergent interference to improved compatibility with downstream applications.
Protease Inhibitors and Phosphatase Inhibitors
Ready-to-use, inhibitor cocktails (not tablets) and individual purified formulations of protease inhibitors and phosphatase inhibitors to protect proteins from degradation during cell lysis and extraction.
Detergents for Cell Lysis
High-purity, low-peroxide, surfactant solutions and detergent solids for use in cell lysis reagent formulation, protein solubilization procedures, wash buffers for ELISA, and other protein research methods.
Stabilizing, Solubilizing and Refolding Reagents
Supporting reagents and kits to stabilize functional enzymes and proteins for storage, solubilize inclusion bodies for recombinant protein purification, and optimize buffer conditions for refolding denatured protein structures.
Enzymes for Cell Lysis
Formulations of enzymes such as lysozyme, deoxyribonuclease I (DNase I) and micrococcal nuclease (MNase) to aid and facilitate cell lysis, protein extraction, subcellular fractionation, organelle isolation and chromatin IP.
Subcellular Fractionation Kits
Reagent-based kits optimized for stepwise separation, enrichment and extraction of proteins from different cell fractions; isolate cytoplasmic, membrane, nuclear, chromatin-bound and cytoskeletal proteins for downstream analysis.
