Protein Affinity Purification

Agarose beads and other resins for specific purification of proteins and other target molecules, kits for immunoprecipitation (IP), activated resins for covalent immobilization of ligands, and columns for packing affinity resins.

Our prepared and activated column chromatography resins, purification kits and immobilization supports provide a complete set of tools to accomplish any protein affinity ligand technique. No other product line delivers a broader range of protein research tools and more years of affnity purification experience. Choose from our wide selection of resin types, kit assemblies and package sizes, or contact our technical support scientists for advice about how to design customized applications.

Learn more:
Immunoprecipitation Kit Selection Guide »
Affinity Purification Methods »

Affinity Resins for Protein Immobilization

Agarose beads and other porous resins activated for chemical attachment of affinity ligands (antibodies, glycoproteins, fusion proteins, peptides) to create affinity supports for purification based on specific binding with the immobilized molecule.

 

Magnetic Affinity Separation Particles

Paramagnetic particles and activated affinity purification beads for benchtop immunoprecipitation procedures and automated protein separation workflows based on antibody, streptavidin, GST and titanium dioxide ligands.

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Biotin-Binding Affinity Resins and Beads

Avidin and streptavidin agarose beads and resins to bind, immobilize and purify biotinylated antibodies and other ligands with high specificity for the avidin-biotin interaction in small- or large-scale affinity purification applications.

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Other Immobilized Affinity Ligands

Agarose beads and other resins with specialized immobilized ligands for diverse affinity methods (lectins, metal ions, ribonucleotides, heparin, etc.). Obtain 1000- to 10,000-fold purification of target molecules from crude mixtures.

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Fusion Protein Purification

Nickel-NTA and cobalt immobilized metal affinity chromatography (IMAC) resins, columns and kits for His-tagged protein purification; Glutathione agarose beads and kits for GST-tagged protein purification.

 

Post-Translational Modification Enrichment Kits

Purification resins to isolate and enrich for sample proteins that contain specific post-translational modifications (PTM) including glycosylation (glycoproteins), ubiquitin (poly-ubiquitinated proteins), and phosphorylation (phosphoproteins).

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Immunoprecipitation and Pull-Down Kits

Several varieties of immunoprecipitation (IP), co-immunoprecipitation (Co-IP) and pull-down assay kits optimized for different sources and types of antibody or other protein affinity systems such as biotinylated and GST-tagged proteins.

 

Spin Columns and Gravity-flow Columns

Different sizes and types of empty purification columns and filter spin columns for packing desalting resins, agarose beads or other affinity supports to use in microcentrifuge or gravity-flow chromatography procedures.

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