Crosslinking and Protein Modification
Reagents to modify proteins by crosslinking, fragmenting, denaturing, reducing disulfides, or attaching various prosthetic groups (e.g. PEGylation) to allow manipulation and study of protein function and interactions in any environment.
When your protein research requires chemical crosslinking, protein conjugation or peptide modification, you can depend on us to have exactly the reagent you need. We offer nearly 100 Pierce* Crosslinkers for conjugating proteins to each other, to other molecules or to derivatized surfaces. These include several patented or exclusive reagents and modifications to improve reagent solubility and facilitate specialized approaches for protein interaction analysis. We also offer specialized pegylation reagents with defined chain lengths, a variety of amino acid modification reagents, reductants such as the odorless TCEP reducing agent, and both soluble and immobilized proteases for digesting nearly any protein.
Learn more:
Crosslinking and Protein Modification Methods »
Chemistry of Crosslinking »
Protein Crosslinking Applications »
Crosslinking Reagents
Chemical crosslinkers and bioconjugation reagents for covalent protein crosslinking techniques to conjugate antibodies, immobilize ligands, attach haptens to carrier proteins, and stabilize folded protein structures and protein interaction complexes.
Proteases and Protein-Cleaving Reagents
Purified and agarose-immobilized proteases for enzymatic proteolysis (cleavage or digestion) of proteins to facilitate amino acid sequencing, peptide analysis and polypeptide structural characterization.
Modification Reagents for Amino Acids
Chemical agents to modify amino acid side chains on proteins and peptides in order to alter native charges, block or expose reactive binding sites, inactivate functions, or change functional groups to create targets for crosslinking and labeling.
Protein Denaturants and Chaotropes
Chaotropic and denaturing chemical agents, including urea and guanidine hydrochloride, to disrupt water interactions and promote hydrophobic protein and peptide solubilization, elution, refolding and structural analysis.
Pegylation Reagents
Activated linear and branched derivatives of polyethylene glycol (PEG) for pegylation and PEG-modification of peptides and proteins via primary amines and sulfhydryl groups to increase solubility, prolong stability and reduce immunogenicity.
Reducing Agents for Protein Disulfides
Purified powders, convenient solutions and solid-phase resins of disulfide reducing agents, including DTT, BME and TCEP, for stabilization of free sulfhydryls (cysteines) and reduction of disulfide bonds in peptides and proteins.
