Affinity Resins for Protein Immobilization
Agarose beads and other porous resins activated for chemical attachment of affinity ligands (antibodies, glycoproteins, fusion proteins, peptides) to create affinity supports for purification based on specific binding with the immobilized molecule.
When your affinity purification experiment depends on a specific ligand or antibody that only you possess, you can easily prepare your own affinity resin by immobilizing the molecule with one of these immobilization techniques. Use the same high-quality coupling resins that we use to manufacture our many affinity purification kits.
Affinity Purification Methods »
Activated agarose beads and other resins for covalent immobilization of affinity ligands through primary amines (N-terminus and lysine in proteins) based on aldehyde and NHS-ester chemistries that are superior to cyanogen bromide (CNBr) activation.
Iodoacetyl-activated agarose beads, resins and kits for covalent attachment of cysteine-terminated peptides, antigens and other sulfhydryl-containing molecules for use in antibody purification and protein affinity purification.
Hydrazide-activated agarose beads and polyacrylamide resins for covalent immobilization of glycoproteins (polyclonal antibodies) and other aldehyde chemicals and periodate-activated polysaccharides and glycosylated molecules for affinity purification.
Unusual supports (solid polystyrene beads) and activated resins for specialized molecular immobilization techniques, including crosslinking orientation and formaldehyde-mediated coupling through activated hydrogen atoms.
Diaminodipropylamine (DADPA) activated agarose beads and polyacrylamide resins and kits to immobilize carboxylate molecules and C-termini of peptides via crosslinking with the carbodiimide crosslinker EDC for affinity purification applications.