Subcellular Fractionation Kits
Reagent-based kits optimized for stepwise separation, enrichment and extraction of proteins from different cell fractions; isolate cytoplasmic, membrane, nuclear, chromatin-bound and cytoskeletal proteins for downstream analysis.
Are you investigating the expression, activation or interactions of proteins that are associated with particular cell compartments? We've developed innovative approaches to extract, fractionate and enrich classes of proteins from a variety of cellular compartments. Isolate nuclear proteins for EMSA (gel shift assays). Solubilize hydrophobic membrane proteins. Purify cell surface proteins to identify protein receptors and orientations.
Cell Fractionation and Organelle Isolation »
Thermo Scientific* Mem-PER Eukaryotic Membrane Protein Extraction Kit enables small-scale solubilization and enrichment of hydrophobic membrane-associated proteins in a simple reagent-based procedure.
The Thermo Scientific* Mem-PER Plus Membrane Protein Extraction Kit enables small-scale solubilization and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure.
Thermo Scientific* NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours.
Thermo Scientific* Cell Surface Protein Isolation Kit is a complete set of reagents for selective biotinylation and subsequent purification of mammalian cell surface proteins to the exclusion of intracellular proteins.
The Thermo Scientific* Pierce Tissue Strainer fits standard 15mL conical tubes for quick separation of cells or cell lysate from tissue debris through a 250µm mesh filter after mechanical disruption using gravity or gentle centrifugation.
The Thermo Scientific* Subcellular Protein Fractionation Kit for Cultured Cells enables segregation and enrichment of proteins from five different cellular compartments.
The Thermo Scientific* Subcellular Protein Fractionation Kit for Tissue includes all reagents and tissue strainers required to perform cell fractionation and enrichment of proteins from five different cellular compartments within 2 hours.