Reducing Agents for Protein Disulfides
Purified powders, convenient solutions and solid-phase resins of disulfide reducing agents, including DTT, BME and TCEP, for stabilization of free sulfhydryls (cysteines) and reduction of disulfide bonds in peptides and proteins.
Our collection of protein-grade formulations and convenient packages of disulfide reductants.
Learn more:
Crosslinking and Protein Modification Methods »
Protein Electrophoresis »
Pierce* 2-Mercaptoethanol
Thermo Scientific* Pierce 2-Mercaptoethanol (2-ME), also called beta-mercaptoethanol (b-ME), is a mild reducing agent for cleaving protein disulfide bonds.
Pierce* 2-Mercaptoethylamine·HCl
Thermo Scientific* Pierce 2-Mercaptoethylamine-HCl, commonly abbreviated 2-MEA, is a mild reductant that is often used to selectively reduce hinge-region disulfide bonds in antibodies.
Pierce* Bond-Breaker* TCEP Solution, Neutral pH
Thermo Scientific* Bond-Breaker TCEP Solution, Neutral pH is a stable, 0.5M solution of the thiol-free, phosphine-based TCEP compound, useful as a 10X stock for addition to SDS-PAGE sample loading buffers to reduce protein disulfide bonds.
Pierce* Cysteine·HCl
Thermo Scientific* Pierce Cysteine-HCl is useful as a sulfhydryl standard or an additive in protein refolding experiments.
Pierce* DTT (Dithiothreitol)
Thermo Scientific* Pierce DTT (Dithiothreitol) is offered as 7.7mg x 48-tube microplates for convenient preparation of 100µL of 0.5M stocks for addition to protein samples for effective reduction of disulfide bonds.
Pierce* Immobilized Reductant Column
Thermo Scientific* Pierce Immobilized Reductant Column contains beaded resin to which an thiol-based reducing agent has been immobilized to enable solid-phase reduction of peptide and protein disulfide bonds.
Pierce* Immobilized TCEP Disulfide Reducing Gel
Thermo Scientific* Pierce Immobilized TCEP Disulfide Reducing Gel is Tris(2-carboxyethyl)phosphine that is covalently immobilized to beaded agarose resin to enable efficient reduction of peptide and protein disulfide bonds.
Pierce* TCEP·HCl
Thermo Scientific* Pierce TCEP-HCl is pure, crystalline Tris(2-carboxyethyl)phosphine hydrochloride, a thiol-free compound that is highly effective at reducing protein and peptide disulfide bonds, as well as being odorless and stable.
