Carboxyl-to-Amine Crosslinkers
Carbodiimide crosslinking reagents DCC and EDC (EDAC) for conjugation of carboxyl groups (glutamate, aspartate, C-termini) to primary amines (lysine, N-termini); also N-hydroxysuccinimide (NHS) for stable activation of carboxylates for amine-conjugation.
Our premium line of Pierce* Crosslinkers for conjugating proteins and peptides via primary amines (N-termini) and carboxyls (C-termini).
Learn more:
Crosslinkers at a Glance »
Crosslinker Selection Guide »
Crosslinking and Protein Modification Methods »
Pierce* DCC
Thermo Scientific* Pierce DCC is dicyclohexylcarbodiimide, an organic-soluble crosslinker that activates carboxyl groups for spontaneous peptide-bond formation with primary amines.
Pierce* EDC
Thermo Scientific* Pierce EDC is a water-soluble carbodiimide crosslinker that activates carboxyl groups for spontaneous reaction with primary amines, enabling peptide immobilization and hapten-carrier protein conjugation.
Pierce* NHS
Thermo Scientific* Pierce NHS is pure N-hydroxysuccinimide, the essential compound needed to prepare amine-reactive NHS-ester crosslinkers or to enhance conjugation efficiencies when using the carbodiimide EDC.
Pierce* Sulfo-NHS
Thermo Scientific* Pierce Sulfo-NHS is pure N-hydroxysulfosuccinimide, the essential compound needed to prepare water-soluble, amine-reactive NHS-ester crosslinkers or to enhance conjugation efficiencies when using the carbodiimide EDC.
