Proteases and Protein-Cleaving Reagents
Purified and agarose-immobilized proteases for enzymatic proteolysis (cleavage or digestion) of proteins to facilitate amino acid sequencing, peptide analysis and polypeptide structural characterization.
We offer both standard and premium purified proteases, as well as innovative proteolysis-based kits for mass spectrometry applications and other analysis methods.
Protease Selection Guide »
Crosslinking and Protein Modification Methods »
Protease Inhibitors »
Thermo Scientific* Pierce Carboxypeptidase Y is a protease with that is able to cleave (release) any terminal amino acid including proline from the C-terminus of peptides; the immobilized form allows sample separation after treatment.
Thermo Scientific* Pierce Factor Xa is a serine endopeptidase composed of two disulfide-linked subunits, whose specificity makes it particular useful in cleaving 6xHis tags from engineered His-tagged fusion proteins.
Thermo Scientific* Pierce Human Rhinovirus (HRV) 3C Protease is a recombinant cysteine protease used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage.
Thermo Scientific* Pierce Immobilized Ficin consists of a sulfhydryl-specific protease (ficin) that has been immobilized onto beaded agarose resin to enable controlled antibody fragmentation, especially of mouse IgG1.
Thermo Scientific* Pierce Immobilized Papain consists of a cysteine-endopeptidase (papain) that has been immobilized onto beaded agarose resin to enable generation and purification of Fab fragments from antibodies.
Thermo Scientific* Pierce Immobilized Pepsin consists of an acidic endopeptidase (pepsin) that has been immobilized onto beaded agarose resin to enable generation and purification of Fab and F(ab')2 fragments from antibodies.
Thermo Scientific* Pierce In-Gel Tryptic Digestion Kit provides a complete set of reagents for digesting and recovering peptide fragments from approximately 150 digestions on stained protein bands excised from SDS-PAGE.
Thermo Scientific* Pierce In-Solution Tryptic Digestion and Guanidination Kit contains proteomics-grade trypsin, buffers and reagents to alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry.
These Thermo Scientific* Pierce Proteases are small, convenient packages of individual, purified highly-specific proteases for use in various combinations to obtain better sequence coverage and protein identification for mass spectrometry.
Thermo Scientific* Pierce Proteinase K is a protease that cleaves at the carboxyl side of aliphatic, aromatic or hydrophobic residues and is commonly used to digest and inactivate DNase and RNase during in nucleic acid purification.