Life Science ResearchLife Science Research ›  Protein BiologyProtein Biology ›  Crosslinking and Protein ModificationCrosslinking and Protein Modification ›  Pegylation ReagentsPegylation Reagents › Pierce* Carboxy-PEG-Lipoamide Compounds
false
ThermoScientific
-1
 

Pierce* Carboxy-PEG-Lipoamide Compounds

Thermo Scientific* Pierce CL(PEG)12, or carboxy-PEG12-lipoamide, is a carboxyl- and bidentate thiol-terminated compound that contains a 4-unit polyethylene glycol (PEG) spacer and is used to modify quantum dots, and silver or gold surfaces and particles.
 
 
 
 
 
CL(PEG)12 is a bidentate thiol-terminated and carboxylic acid pegylation reagent. The PEG compound has a defined molecular weight and spacer length and is useful for modifying surfaces such quantum dots, self-assembled monolayers and magnetic particles. Functionalization of solid surfaces with this polyethylene glycol reagent provides carboxylate handles for protein immobilization via EDC crosslinking and significantly reduces nonspecific protein binding and provides.

Highlights:

  • Metal-binding – terminal bidentate thiol reacts spontaneously with silver, gold and other metal surfaces to form strong dative bonds
  • Carboxylate – terminal carboxylic acid group allows conjugation to amine-containing affinity ligands using carbodiimide (EDC) and Sulfo-NHS crosslinking chemistry
  • Polyethylene glycol – PEG groups are flexible, non-immunogenic, hydrophilic, and significantly reduce nonspecific binding of surfaces for protein methods
  • Spacer arm – 12-unit PEG spacer makes the reagent nearly 56 angstroms long, minimizing steric hindrance for protein immobilization and binding assays
Specifications:

  • Alternative name: Carboxy-PEG12-Lipoamide
  • Chemical name: 45-(1,2-dithiolan-3-yl)-41-oxo-4,7,10,13,16,19,22,25,28,31,34,37-dodecaoxa-40-azapentatetracontan-1-oic acid
  • Chemical formula: C35H67NO15S2
  • Molecular weight: 806.03
  • Spacer arm: 55.5 angstroms
  • Form: yellow solid
  • Solubility: dissolve in DMSO or DMF for use
The use of ML(PEG)4 with CL(PEG)12 in surface modification can form a hydrophilic lawn of methyl ether-terminated PEGs with periodic exposed carboxy -containing PEGs. The exposed carboxylic acid groups can be coupled to affinity ligands using the carbodiimide coupling reaction with EDC and sulfo-NHS.

Typical PEG reagents contain heterogeneous mixtures of different PEG chain lengths; however, our PEG reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimizing modification applications.


 
 
 
 
Help image

Need Help?


Have a Question?
We're here to help you.

Select Country for Contact Info This website provides global contact information for our products and services. When you select a Contact Country, the contact information that is displayed by default will be for this country.



  • Sales
 

In The Spotlight
uspgh-zcom-p3-leg7.fishersci.com:80