Deparffinization is accomplished by melting the paraffin at the high temperature used in HIER (over 90°C) along with emulsification of the paraffin by detergent. Formaldehyde fixation impairs or totally destroys the immunoreactivity of many antigens and epitopes. The negative effect of formaldehyde fixation can be reversed successfully with enzymatic digestion for some markers but not for others. Non-enzymatic epitope unmasking techniques have been introduced to improve the immunoreactivity of many antigens in formaldehyde fixed tissues. Heat-Induced Epitope Retrieval (HIER) in Citrate, EDTA or Tris-HCl buffer has been found to improve the reactivity of many antibodies in formalin-fixed tissues.
- Available in Citrate, EDTA or Tris-HCl at various pH levels
- 100X stock solution
- Dilute 100-fold with distilled water before use
Storage Conditions: Store at room temperature. Stable for up to 18 months. Contains no preservatives. For periods longer than three months, store at 2°-8°C.
Compatible with: Epitope Retrival Solutions are designed for use with Pretreatment Module*. Determination of use conditions in any other instrument is the responsibility of the user.