Life Science ResearchLife Science Research ›  Cell BiologyCell Biology ›  Cell Culture Media, Sera, and ReagentsCell Culture Media, Sera, and Reagents ›  MediaMedia ›  Stem Cell MediaStem Cell Media › HyClone AdvanceSTEM Cell Culture Media
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ThermoScientific
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HyClone AdvanceSTEM Cell Culture Media

Thermo Scientific* HyClone AdvanceSTEM Cell Culture Media is 'optimized for growth of embryonic cell cultures.
Providing optimal culture conditions can be one of the greatest challenges in stem cell research, particularly in keeping cells in an undifferentiated state or directing differentiation when desired. HyClone AdvanceSTEM line of stem cell culture products can help you meet those challenges. Classic culture conditions for 129-derived murine embryonic stem cells (mESCs) include culturing in medium containing DMEM, FBS, LIF and co-culturing with primary mouse embryonic fibroblasts (MEFs). Pluripotent mESCs have a very distinct morphology when cultured under these conditions; growing as tightly clustered colonies with smooth phase bright borders. When co-cultured in ES cell media with a mouse embryonic fibroblast (MEF) feeder layer, these stem cell cultures are suitable for electroporation, expansion, or further experimentation.
 
 
 
 
Part Number Description   Quantity
SH30822.01 IMDM4SC; 500mL
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SH30822.02 IMDM4SC; 1L
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SH30824.01 DMEM4SC; 500mL
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SH30824.02 DMEM4SC; 1L
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SH30870.01 Low Osmo DMEM; 500mL
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SH30870.02 Low Osmo DMEM; 1000mL
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Please Note: Availability may vary by country. Show All
 
  • ES cell-qualified with Thermo Scientific HyClone's ES Screened FBS—no prescreening required
  • Mycoplasma free and sterility tested
  • DMEM4SC is formulated without L-Glutamine or sodium pyruvate and is ideal for use with 129 ES cells
  • IMDM4SC contains no L-Glutamine and supports excellent growth of B6 ES cells
  • AdvanceSTEM Serum Replacement developed for growth of undifferentiated embryonic cells in culture minimal or no requirement for fetal bovine serum
  • AdvanceSTEM Low Osmo DMEM is basal medium developed to support growth and maintenance of ES cells in an undifferentiated state

Storage Conditions: Store at 2° to 8°C

 
 
 
 
 
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