HyClone Hygromycin B

Thermo Scientific* HyClone Antibiotics are used to control/destroy destructive microorganisms, such as bacteria, fungi and higher eukaryotic cells

HyClone offers a selection of quality antibiotics to control bacterial, yeast, fungi, and mycoplasma contaminations, as well as premium selection antibiotics. The introduction of laminar-flow hoods coupled with good aseptic techniques has greatly reduced the necessity to add antibiotics in most cell culture protocols. However, antibiotics are still routinely added to cell culture media. Improving aseptic techniques and limiting antibiotic use is desirable, as overuse of antibiotics can encourage the development of antibiotic resistant organisms, hide the presence of a low level contamination that can become fully operative if 1) the antibiotics are removed, 2) the culture conditions change, or 3) if resistant strains develop. They can also have anti-metabolic effects that could cross-react with mammalian cells. We recommend that the use of antibiotics be limited and removed from the culture if possible. When antibiotics are used for long periods of time, parallel cultures should be maintained free of antibiotics.