Life Science Rese...Life Science Research ›  Cell BiologyCell Biology ›  High Content Fluo...High Content Fluorescence Imaging ›  Reagent- and Cell...Reagent- and Cell Line-Based Assays ›  Antibody- and Dye...Antibody- and Dye-Based Assays › Cellomics* Cell Viability HCS Reagent Kit
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ThermoScientific
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Cellomics* Cell Viability HCS Reagent Kit

The Thermo Scientific* Cellomics Cell Viability HCS Reagent Kit is for cellular imaging and quantitation of live cells, dead cells and total cell counts.
 
 
 
 
 
The fixed end-point assay is based on fluorescent staining of cells grown on standard high-density microplates. Specific fluorescent probes effectively measure differences between treated and nontreated plates of cells with respect to the cell viability parameters. The procedure is optimized for use with the Thermo Scientific ArrayScan* Reader and Cell Viability BioApplication software to provide automated plate handling, focusing, cell image acquisition, and analysis of cell viability.

Features:

  • Quantifies drug candidate toxicity with state-of-the-art precision and speed on the ArrayScan High Content Reader
  • Cost-effective, streamlined kit format simplifies protocols and sample/reagent preparation
  • Assays can be performed alone or in combination with other High Content assays for multifaceted drug screening
  • DeadDye* and VitalDye* fluorescent probes offer sensitivity, quantitative and qualitative screening results without radioactivity

Required Accessories: Fluorescence microscope, Thermo Scientific Cellomics ArrayScan VTI or other HCS reader
For a more detailed description of the image processing algorithm, see the Cell Viability Application Guide that accompanies the software.
The core reagents supplied include DeadDye* and VitalDye* in addition to Hoechst dye for identifying nuclei. When cells are dead or dying, plasma membranes leak and become permeable to large macromolecules. In this state, DeadDye enters the cell and is visualized by its red fluorescence emission. VitalDye, a membrane-permeable fluorescent probe, operates as a live-cell indicator. Soluble cytoplasmic enzymes cleave VitalDye, trapping its fluorescent moiety inside intact cells. Live cells stained with VitalDye are distinguished from dead cells by their green fluorescence emission. Identification of the entire cell population is achieved using Hoechst Dye, a membrane-permeable nucleic acid stain emitting in the blue fluorescence channel. DeadDye and VitalDye may be used separately or in combination to quantify dead cells, live cells or both cell populations.

One application of this screen is the determination of the LD50 or the concentration dependence of the lethality of a compound. Cell viability is only a single aspect of drug candidate screening. The ArrayScan High Content Reader addresses this by performing several assays simultaneously on the same cells and in the same screening run. Insightful determinations of cell viability in conjunction with other cellular targets, pathways, and organelle changes are fully automated and accessible through the ArrayScan High Content Reader and the associated BioApplications.


 
 
 
 
 
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