The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation or toxic phenol-chloroform extractions.RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications including RT-PCR, RT-qPCR, Northern blotting and other RNA-based analyses.
- Universal—can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, bacteria
- Efficient—high yields of total RNA
- Fast—15 minutes after lysis step
- Pure—an A260/280 ratio > 1.9
- Convenient— spin columns are capped and assembled with collection tubes
Includes: Proteinase K, Lysis Buffer, Wash Buffer 1 (concentrated), Wash Buffer 2 (concentrated), Water, nuclease-free, GeneJET RNA Purificatio, Columns (pre-assembled with Collection Tubes), Collection Tubes (2mL), Collection Tubes (1.5mL), Detailed Protocol
Recommended for: Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including: RT-PCR, RT-qPCR, Northern blotting, nuclease protection assay
References:
Chomczynski, P. and Sacchi. N., Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction, Anal. Biochem., 162, 156–159, 1987. Boom, R., Sol C.J.A., Salimans, M.M.M. Jansen, C.L. Dillen, P.M.E.W. an
