HyClone* AdvanceSTEM HEPES Buffer, ES Cell Qualified

Thermo Scientific* HyClone AdvanceSTEM ES Qualified HEPES is better at maintaining physiological pH despite changes in carbon dioxide as compared to bicarbonate buffers.

Providing optimal culture conditions can be one of the greatest challenges in stem cell research, particularly in keeping cells in an undifferentiated state or directing differentiation when desired. HyClone AdvanceSTEM line of stem cell culture products can help you meet those challenges. Classic culture conditions for 129-derived murine embryonic stem cells (mESCs) include culturing in medium containing DMEM, FBS, LIF and co-culturing with primary mouse embryonic fibroblasts (MEFs). Pluripotent mESCs have a very distinct morphology when cultured under these conditions; growing as tightly clustered colonies with smooth phase bright borders. When co-cultured in ES cell media with a mouse embryonic fibroblast (MEF) feeder layer, these stem cell cultures are suitable for electroporation, expansion, or further experimentation.