The kit uses RevertAid Reverse Transcriptase which has lower RNase H activity, compared to AMV reverse transcriptase. The recombinant RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55°C. The oligo(dT)18 anneals selectively on the poly(A) tail of mRNA. Random hexamer primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5'-end regions of mRNA or cDNA synthesis using RNA without poly(A) tail, e.g., micro RNAs. Gene-specific primers may also be used with the kits. The first strand of cDNA can be directly used as a template in PCR (see RT-PCR protocol), real-time PCR or in second strand cDNA synthesis (see protocol).
- Full-length first strand cDNA up to 13kb
- Increased reaction temperatures in the range of 42° to 50°C
- Supplied with the recombinant RiboLock RNase Inhibitor
- Complete—oligo(dT)18 and random hexamer primers included with the kit
Recommended for: First strand cDNA synthesis for RT-PCR and real-time RT-PCR (1, 2); Construction of full length cDNA libraries; Generation of probes for hybridization; aRNA synthesis.
References:
Schmidt, A., Su, Y.H., et al., UPS1 and UPS2 from Arabidopsis Mediate High Affinity Transport of Uracil and 5-Fluorouracil, The Journal of Biological Chemistry, vol. 279, 43, 44817–44824, 2004., Papavinasasundaram, K.G., et al., Deletion
