It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter.
- Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)
Recommended for: Synthesis of unlabeled and labeled RNA that can be used:for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.
Consensus promoter sequences: AATTAACCCTCACTAAA G GGAGA - T3 TAATACGACTCACTATA G GGAGA - T7 ATTTAGGTGACACTATA G AAGNG - SP6 The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively (9).
Krieg, P.A., Melton, D.A., Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs, Nucleic Acids Res., 12, 70577070, 1984., Melton, D.A., Injected antisense RNAs specifically block messenger RNA tr