The presence of SDS eliminates DNA-protein interactions and prevents gel-shifts. The high concentration of EDTA protects DNA from degradation by metal-dependant nucleases (see recommendations for use).
- 1% SDS eliminates DNA-protein interactions, prevents appearance of additional bands due to annealing of DNA molecules with cohesive ends
- 100mM EDTA inhibits metal-dependent nucleases
Recommended for: Analysis of DNA samples with high amounts of DNA binding proteins; Kinetic experiments; DNA agarose gel analysis after DNA restriction digestions, ligation or dephosphorylation reactions.