LTQ XL* Linear Ion Trap Mass Spectrometer

• Unparalleled sensitivity and unmatched MSⁿ spectral quality
• Pulsed Q Dissociation (PQD) for generating more fragments and extending the low mass range
• Electron Transfer Dissociation (ETD) option provides sequence information not available from conventional methods
• High Resolution Isolation (HRI) enables isolation of isobaric mass peaks for cleaner MSⁿ spectra
• Ultra-fast polarity switching for high sensitivity analysis of unknowns
• Automated Data Dependent neutral loss for PTM and biotransformation analyses
• Excellent charge state determinations with Ultra ZoomScan*
• Upgradeable to LTQ Orbitrap XL accurate mass technology

• Accela High-Speed LC System (IQLAAAGABHFAAAMAAG)
• Surveyor Plus HPLC System (IQLAAAGAAOFAFHMALP)
• LTQ FT Ultra Hybrid Mass Spectrometer (IQLAAEGAAPFADAMAII)
• LTQ Orbitrap Hybrid Mass Spectrometers ( IQLAAEGAAPFADBMAIJ)
• ETD Fragmentation, Electron Transfer Dissociation (IQLAAEGABBFACCMAFJ)
• LCquan 2.5.5 ( SID-LCQUAN25)
• Mass Frontier Spectral Interpretation Software (IQLAAEGABOFAGUMZZZ)
• MetWorks Automated Metabolite Identification Software (IQLAAEGABOFAGTMZZZ)
• PEAKS Studio 4.0 (SID-PEAKS40)
• ProMass Deconvolution 2.5 (OPTON-20875)
• Pulsed-Q Dissociation (PQD) (IQLAAEGABBFAGQMZZZ)
• SIEVE Automated Label-Free Differential Expression Software (INQSOF012/IQLAAEGABSFAHSMAPV)

• Protein identification and quantitative differential expression analysis
• Biomarker studies and PTM identification
• Metabolite identification and quantification
• Tissue imaging
• High-speed LC methods

Ion Max* API Source

• Enhanced sensitivity and ruggedness
• Sweep Gas* reduces chemical noise
• 60° interchangeable ion probe orientation
• Removable metal ion capillary tube provides vent-free maintenance

Transfer Ion Optics

• Advanced ion guides
• High stability and ion transmission efficiency

2D Linear Quadrupole Ion Trap Mass Spectrometer

• Optimized analyzer dimensions
• Regulated helium flow for stable performance
• Automatic system calibration
• High-efficiency radial ion ejection

Vacuum System

• Differentially-pumped vacuum system to 10^-5 Torr
• Split-flow turbomolecular pump controlling vacuum in three regions
• Dual rotary vacuum pump configuration
• High-vacuum aluminum analyzer chamber

Detection System

• Proprietary dual conversion dynode detector
• Two off-axis continuous dynode electron multipliers with extended dynamic range
• Digital electronic noise discrimination

Integrated Divert Valve

• Fully-automated data system control enables user to divert the solvent front, gradient end point and any other portion of the HPLC run to waste
• User-definable default state of the valve, either “to waste” or “to source”

Integrated Syringe Pump

• Syringe Pump allows automated infusion under data system control

• ESI probe compatible with liquid flowrates of <1µL/min to 1mL/min, without splitting
• APCI source compatible with liquid flowrates of 50µL/min to 2mL/min, without splitting
• APCI/APPI source compatible with liquid flowrates of 50µL/min to 2mL/min, without splitting
• Electron Transfer Dissociation (ETD) enabled
• vMALDI* ion source combines the speed of MALDI with the sensitivity and selectivity of MS/MS
• Nanospray source supports static packed tip and dynamic nanospray experiments, compatible with liquid flowrates of 50nL/min† to 2µL/min
• Metal needle option for high- and low-flow analyses

†Lower limit is dependent on gauge of needle used

Software Features

Data System

• Xcalibur processing and instrument
• control software
• LCQUAN* 2.5 quantification package
• Microsoft* Office XP software package
• Microsoft Windows* XP operating system
• High-performance PC with Intel* Pentium* microprocessor
• High-resolution LCD color monitor

Scan Functions

• Full-scan mass spectra for sensitive analyses and rapid screening of unknown compounds
• Selected Ion Monitoring (SIM) for selected ions for target compound analysis
• Full-scan product ion spectra at sensitivities higher than any ion trap mass spectrometer
• Selected Reaction Monitoring (SRM) for a traditional LC/MS/MS quantitative analytical experiment
• MSⁿ for multi-stage MS experiments to probe the structure of ions
• ZoomScan,™ a high-resolution, full-range scan to resolve isotopic envelopes often used for charge state determination of peptides and ionization state determination of organometallics
• Ultra ZoomScan for ultimate resolution
• TurboScan*, an ultra-fast scan to improve signal-to-noise and sampling rate

Exclusive Technologies

• Pulsed Q Collision Induced Dissociation (PQD) enables trapping of low mass fragment ions
• Electron Transfer Dissociation (ETD) enabled capability delivers peptide sequence information not available from conventional methods
• High Resolution Isolation (HRI) allows for the separation of an isobaric interfering species down to 0.3 Da or for isolation of a thermally labile compound
• Unique, proprietary Automatic Gain Control (AGC*) ensures that the ion trap is always filled with the optimum number of ions for any scan type
• Dynamic Exclusion* allows acquisition of MS/MS and MSn spectra from lower intensity ion species
• WideBand Activation* generates more structurally informative spectra
• Normalized Collision Energy* compensates for the mass-dependent energy deposition characteristics of ion trap mass spectrometers in MS/MS experiments, providing reproducible data from instrument to instrument
• Stepped Normalized Collision Energy allows for the variation of the collision energies in an experiment
• Multistage Activation generates combination MS/MS spectra and MS3 spectra based on a user defined neutral loss

Advanced Data Dependent Experiments

• Data Dependent features trigger acquisition of MSⁿ spectra only when a compound of interest is detected
• Isotopic Data Dependent scanning software triggers MS/MS scanning only when a specified isotopic pattern is detected
• Triple Play determines the charge state and MS/MS scan range of a multiply charged ion
• Nth Order Triple Play allows the number of ions undergoing a Triple Play to be defined
• Data Dependent Neutral Loss (NL) MS³ triggers MS³ scan only on the MS/MS product ions with the pre-defined neutral loss to identify and characterize metabolites and post-translational modifications
• Ion Mapping* automatically generates a 3-dimensional MS/MS map, yielding product ion, precursor ion, and neutral loss information
• Ion Mapping Browser Software displays data generated by Ion Mapping experiments
• Data Dependent Ion Tree performs MSⁿ experiments on up to 25 species
• Data Dependent Zoom Map generates sequential MS/MS experiments using a ZoomScan for charge state determination prior to each MS/MS experiment
• MSⁿ Browser software displays data generated by Data Dependent Ion Tree and Ion Mapping experiments

Optional Application-Specific Software

• MetWorks* – automated metabolite identification using spectral trees
• Mass Frontier* – spectral interpretation and classification software to identify unknowns
• PEAKS – powerful, easy de novo sequencing
• ProMass* Deconvolution – intact protein analysis
• SIEVE* – automated label-free differential expression of proteins and peptides
• ProSight PC* – top-down protein identification

System Specifications

MS/MS Sensitivity

Electrospray Ionization (ESI) – A loop injection of 2µL of a 125fg/µL solution of reserpine (250 femtograms total sample) at a flow of 400µL/min of 50% isopropyl alcohol/50% water will produce a minimum signal-to-noise ratio of 100:1, for the transition of the isolated protonated molecular ion at m/z 609 to the largest two product ions, 397 and 448, when the mass spectrometer is operated at unit resolution in the full-scan MS/MS mode, scanning the product ion spectrum from m/z 165 to 615.

Atmospheric Pressure Chemical

Ionization (APCI) – A loop injection of 2µL of a 125fg/µL solution of reserpine (250 femtograms total sample) at a flow of 400µL/min of 50% isopropyl alcohol/50% water will produce a minimum signal-to-noise ratio of 100:1, for the transition of the isolated protonated molecular ion at m/z 609 to the largest two product ions, 397 and 448, when the mass spectrometer is operated at unit resolution in the fullscan MS/MS mode, scanning the product ion spectrum from m/z 165 to 615.

MSn Sensitivity

Electrospray Ionization (ESI)

A loop injection of 2µL of a 125 fg/µL solution of reserpine (250 femtograms total sample) at a flow of 400µL/min of 50% isopropanol/50% water will produce a minimum signal-to-noise ratio of 25:1, for the transition of the unit isolated protonated molecular ion at m/z 609 to the product ion at m/z 397 which is further fragmented to the product ion at m/z 365, when the mass spectrometer is operated at unit resolution in the full-scan MS/MS mode, scanning the product ion spectrum from m/z 165-615.

Installation Requirements

Power

• 230VAC ±10.0%, 50/60Hz, 15A, single-phase, earth-grounded supply dedicated to the instrument
• 120 or 230VAC single-phase, earth-grounded supply for the data system

Gas

• One high-purity (99% pure, flowrate 15L/min) nitrogen gas supply for the API source
• One ultra-high-purity helium gas supply (99.998% pure) with <1ppm each of water, oxygen, and total hydrocarbons for the mass analyzer

Environment

• System averages 2300w (8000 BTU/hr.) output when considering air conditioning needs
• Operating environment must be 15° to 27°C (59° to 80°F) and relative humidity must be 40 to 80% with no condensation
• Optimum operating temperature is 18° to 21°C (65° to 70°F)