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Electron Transfer Dissociation (ETD)

Thermo Scientific* Electron Transfer Dissociation (ETD) is a powerful fragmentation technique that complements traditional collision-induced dissociation (CID).
Unlike traditional CID, which cleaves weakly bound modifications off the peptide backbone, ETD uses reagent gas anions and gas-phase ion/ion chemistry to induce fragmentation along the peptide backbone in a sequence-independent manner, leaving labile modifications linked to the peptide chain. This greatly simplifies identification of the site(s) of modification. ETD produces primarily c- and z-type fragment ions that are an excellent complement to the b- and y-type fragment ions produced by CID. Together, ETD and CID can significantly increase sequence coverage and give added confidence to protein identifications.
 
 
 
 
Part Number Description   Quantity
IQLAAEGABBFACCMAFJ Electron Transfer Dissociation (ETD)
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Electron transfer dissociation is a supremely useful tool for the analysis of post-translationally modified proteins. ETD capability can be added to many Thermo Scientific ion trap and Orbitrap mass spectrometers. Thermo Scientific Proteome Discoverer software includes unique capabilities to help users take full advantage of the information provided by ETD.

  • ETD preserves labile PTMs, allowing straightforward identification of the peptide sequence and the site of modification
  • For top-down analysis, ETD can be combined with proton transfer reaction (PTR) which dramatically simplifies spectra from intact proteins
  • For workflows that include low-mass isobaric mass tags, ETD can be combined with pulsed-Q dissociation (PQD) which eliminates the normal low-mass cutoff
  • ETD can be added to most Thermo Scientific LTQ ion trap and LTQ Orbitrap* hybrid mass spectrometers
  • Thermo Scientific Proteome Discoverer software includes the proprietary Z-Core database search algorithm specifically optimized for ETD spectra
  • Proteome Discoverer can easily merge complementary CID and ETD results to maximize protein sequence coverage

Compatible with:
  • LTQ family of linear ion trap mass spectrometers
  • LTQ Obritrap family of hybrid mass spectrometers
  • Proteome Discoverer software

 
 
 
 
 
 
 
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