1000 Proteins per Hour [pph] Identified from an E. coli Digest - Maximizing Protein Identification on the LTQ Orbitrap Velos

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1000 Proteins per Hour [pph] Identified from an E. coli Digest -
Maximizing Protein Identification on the LTQ Orbitrap Velos

Enormous improvements in mass spectrometry technology over the last few years have allowed for ever deepening analysis of complex proteomes. However, many low abundance proteins remain undetected in current large-scale proteomic analyses due to the limits on the rate at which MS/MS spectra can be acquired. This application note demonstrates and discusses the ability of the newly developed hybrid instrument, the Thermo Scientific LTQ Orbitrap Velos mass spectrometer, to identify more proteins with increased sequence coverage and confidence. Different strategies regarding chromatographic run times and utilizing the instruments various dissociation techniques are compared.