A Simple and Reliable Workflow for Automated Bench Top Protein Estimation

An automated protein estimation work flow using the Thermo Scientific Hydra DT and the Thermo Scientific Pierce 660 nm Protein Assay Reagent is described.

The user friendly Matrix Hydra DT automated liquid handling platform and the ready-to-use Pierce 660 nm Protein Assay Reagent have been combined to offer a solution for rapid mid/high-throughput protein estimation. A comparison of experimental results obtained by manual and automated methods indicates a correlation of >99%, thereby offering a simple bench top method for automated protein estimation.The automated procedure yielded a coefficient of variance (CV) of 1.1%in a 96-well format and a run time of 120 seconds per plate was observed.

Brian Hewson and Tal Murthy, Ph.D., Thermo Fisher Scientific, Hudson, NH, USA

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