Determination of DEHP in Culture Media by GC-MS/MS Using PCI Ammonia

Coupled with MS/MS, the matrix interferences from biological samples were eliminated, enabling quantitation in the low µM levels in culture media.

This application note describes a method of elucidating the target phthalate, bis(2-ethlyhexl) phthalate (DEHP), in culture media by GC-MS/MS using positive chemical ionization (PCI) with ammonia reagent gas.

Jessie Butler, Pat O’Brien, Stacy Crain, Thermo Fisher Scientific, Austin, Texas, USA

Viviane Yargeau, Ph.D., McGill University, Montreal, Canada

AN52282

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