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Welcome to the Cell Culture Café, a science-based community where techniques, tips, and questions concerning cell culture are presented and discussed. Each month, via our interactive webinars, our cell culture experts will explore topics ideally suited to your biggest concerns in the lab.

If you have a suggestion for a future webinar, let us know! We welcome your feedback and want to learn what topics interest you.

So grab a cup of coffee and meet us at the next Cell Culture Café webinar!

Next Webinar:  No more separation anxiety! A research case study: Gentle, non-enzymatic detachment of sensitive cells 

What if you could gently detach even the most sensitive cells without enzymes or scraping, all while maintaining the effectiveness of your process, increasing the ease and speed of detachment and preserving cell surface proteins? Please join research scientist and esteemed colleague, Marten B. Maeß from Friedrich Schiller University – Jena, Germany – as he presents his research showing the application of an innovative surface to enable transfection and non-invasive detachment of macrophages. This research case study can be applied to diverse applications and cell types, offering insights into viable alternatives to your cell detachment methods.

Topics Covered:
  • A research overview: Approaches to transfection and transfection of human macrophages
  • Optimizing macrophage transfection protocol
  • Detachment of cells by temperature reduction
  • Consistent transfection efficiency and preserved functionality of the transfected cells

Our Presenter:
Marten B. Maeß
Research Scientist
Friedrich Schiller University

On-demand webinars

The seven keys to safeguarding your cryopreserved cells

Safeguarding your samples throughout the entire cryopreservation process can be tricky and even unproductive, thanks to protocols that unknowingly undermine your results. Understanding the seven keys to protecting your cryopreserved cells – from selecting the right storage temperatures and proper cooling methods to avoiding common safety hazards when using liquid nitrogen – can equip any lab with the knowledge necessary to ensure sample safety and optimal cryopreservation results. During this webinar, we will discuss each of The Seven Keys along with the benefits you can achieve as a result.

Topics Covered:

  • The big picture: The cryopreservation process
  • What you need to know about cryopreservation temperatures and cooling rates
  • Demystifying the tube selection process and knowing the vital features for sample safety
  • Avoiding critical risks when using liquid nitrogen

Presenter: Jason Xu, Ph.D.    Recorded: March 20, 2014

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Take your Samples for a Spin: Fundamentals of Cell Culture Centrifugation 

Separating cells is a critical step in the dynamic process of cell culture. This webinar will examine the role of centrifugation in the cell culture workflow and explore the different types of centrifuges used to separate cells. We will look at centrifuge requirements and technical considerations for cell culture applications, including selecting the appropriate speed and g-force. State-of-the-art trends in centrifugation will also be discussed. 

Topics Covered:

  • The role of centrifugation in cell culture
  • Centrifuge requirements for cell culture applications
  • Selecting the appropriate speed and g-force for your cell type
  • Trends in cell culture centrifugation

 Presenter: Ileana Place, Ph.D.   Recorded: February 20, 2014

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Avoid the icebergs! Managing sample preparation for cryo storage

Determining the proper sample preparation technique for long-term cryopreservation storage is of paramount importance to all downstream work stemming from the affected samples. During this webinar, we will discuss what happens during the sample preparation process, key concepts to be familiar with and key questions to consider when making decisions about long-term storage sample preparation.

Topics Covered:

  • Sample preparation in relation to long-term cryopreservation storage
  • Different methodologies in sample prep for cryopreservation storage
  • Criteria to account for an optimized sample prep experience

Presenter: Dr. Alex Esmon   Recorded: January 30, 2014

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Maintaining Integrity: Selecting the Right Storage Tube for Downstream Cell Culture

Ensuring storage integrity and traceability of cells is of particular importance for cell culture applications. This webinar will provide detail related to cell preservation and storage considerations, as well as criteria for selecting appropriate storage tubes to meet your downstream cell culture needs.

Topics Covered:

  • Considerations for cell preservation methods
  • Storage tube selection criteria and why these are important to cell culture applications
  • Importance of maintaining sample integrity and traceability through the use of barcoded tubes and complimentary accessories

Presenter: Kiara Biagioni   Recorded: December 12, 2013

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Optimize your cell culture workflow with Thermo Scientific™ peristaltic pumps

Optimizing cell culture processes is often dependant on how the cell culture fluid and media are handled. The proper selection of fluid handling equipment – pumps – can help improve efficiency and production in bio-processing research and production. 

Topics Covered:

  • Application of pumping systems in the cell culture process
  • Tips for preventing contamination in fluid handling systems
  • Optimizing cell growth environments
  • Improving filtration process through improved pumping systems
  • Increasing efficiency of dispensing processes

Presenter: Gregg Johnson    Recorded: October 24, 2013

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Solutions for Optimizing Stem Cell Expansion and Pluripotency

Whether you're focused on cell signaling and development, drug discovery or cell replacement therapies, your investigations require billions of cells. A viable alternative source for these studies is pluripotent stem cells.

Learn how you can accelerate the pace of your discoveries by optimizing pluripotent stem cell cryopreservation and expansion while maintaining pluripotency in both feeder-dependent and feeder-free systems. Integrating Fetal Bovine Serum (FBS) alternatives into your research for improved control and performance

Topics Covered:

  • Promoting healthy stem cell morphology
  • Achieving fast expansion of pluripotent stem cells
  • Maintaining pluripotency
  • Improving stem cell recovery from cryopreservation

Presenter: Amy Sinor-Anderson, PhD    Recorded: September 12, 2013

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Integrating Fetal Bovine Serum (FBS) alternatives into your research for improved control and performance

FBS is the most common supplement used in cell culture based research. Due in part to increased demand, years of drought and economic forces throughout the world, serum supply is increasingly tight.

Discover how you can safeguard your research and budgets from increasingly scarce and expensive FBS with alternatives that are specifically designed to replace FBS and offer improved yield, consistency and cost-savings.

Topics Covered:

  • Determine the best supplementation options for your research
  • Recognize the tradeoffs between FBS and FBS alternatives
  • Save research dollars with high-quality serum-based FBS alternatives

Presenter: Nathan Allen, MA, MBA    Recorded: August 22, 2013

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Choosing the right cold storage for cell culture applications

Provides practical pointers on how to select the best cold storage and sample prep solutions, with an eye to pertinent criteria that can lead to an optimized cell culture experience.

Topics Covered:

  • Cold storage touch points in the cell culture workflow
  • Types of cold storage products: how and what differentiates them
  • Brief discussion of performance characteristics
  • Criteria to consider for an optimized experience

Presenter: Dr. Alex Esmon    Recorded: July 18, 2013

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Surface Selection: Achieving optimal growth and harvesting of your cells

In this webinar, we give an overview of established and novel technologies used to modify cultureware surfaces for optimally growing adherent as well as non-adherent mammalian cells. We characterize the different Thermo Scientific™ Nunc™ surfaces by their adhesion properties and provide examples of how best to select the best culture surface for your application. We also present alternative methods to help improve cell harvesting.

Topics Covered:

  • Different technologies for surface modification improving cell adhesion
  • Overview and characterization of Nunc cell culture surfaces arranged by adhesion properties
  • Examples of how to select the right surface for an application
  • Alternative methods for advanced cell harvesting

Presenter: Dr. Thomas Stelzer    Recorded: May 16, 2013

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Protect You and Your Science - with biological safety cabinets

Effective use of the Class II biological safety cabinet (BSC) protects you and your important work. Explore how BSCs use effective air filtration and controlled airflow to prevent contamination from passing into the BSC.

Topics Covered:

  • How BSC filters are different from most air filters
  • The three airflows of the BSC; inflow, downflow and exhaust
  • Selecting among the common types of Class II BSCs
  • How to get the most out of any BSC
  • Five myths of BSC operation

Presenter: Dave Phillips    Recorded: April 11, 2013

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Choosing the ideal lab water system for your cell culture applications

Topics Covered:

  • How water impurities affect your cell culture experiments
  • Choosing the right water purification system for your applications
  • Latest trends in lab water systems
  • Proper system maintenance

Presenter: Julie Akana    Recorded: March 14, 2013

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Take your Cell Based Assays to the Edge

Topics Covered:

  • Reducing Edge Effects for Full Plate Results
  • Increasing Assay Performance
  • Improving Long-Term Cell Based Kinetics

Presenter: Sarah Verna, Christian Oberdanner    Recorded: January 31, 2013

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Culture Your Cells Using Less Oxygen for Biologically Relevant Results

Topics Covered:

  • History and advances in understanding oxygen concentration
  • Mechanism of cellular oxygen sensing and response
  • Effects of low oxygen (hypoxia) on cultured cells

Presenter: Mary Kay Bates    Recorded: December 6, 2012

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The Last Line of Defense: Cell Culture Contamination and Using Filtration as a Final Preventative Measure

Topics Covered:

  • Understanding and protecting against biological contamination
    • Types, detection, sources and reducing risk
  • How to choose the right filter
  • Types of filter membranes and how they work

Presenter: Dennis Gorlick    Recorded: November 8, 2012

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Cell Culture Contamination: What You Don't Know Can Cost You

Topics Covered:

  • Varied types of contamination and the extent of the problem
  • Reducing contamination in your laboratory
  • How to evaluate CO2 incubator design innovations for contamination prevention

Presenter: Mary Kay Bates    Recorded: October 11, 2012

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