Cell Culture Café
Welcome to the Cell Culture Café, a science-based community where techniques, tips, and questions concerning cell culture are presented and discussed. Each month, via our interactive webinars, our cell culture experts will explore topics ideally suited to your biggest concerns in the lab.
If you have a suggestion for a future webinar, let us know! We welcome your feedback and want to learn what topics interest you.
Next webinar details coming soon!
Until then, grab a cup of coffee and listen to some of our informative past Cell Culture Café webinars ...
Versatile is smart! The power behind culturing cells on a porous membrane
There are times when your cell-based research calls for more than your standard cell culture vessels and dishes. The porous membrane and unique structure of cell culture inserts enables the versatility you need by allowing the attached surface of your cells to be exposed to growth media and different conditions simultaneously— providing easier methods for some procedures, such as cultures that require an air-liquid interface and media feeding.
In this presentation, we examine the possible uses and adaptability of cell culture inserts and review a case study using cell culture inserts to produce an artificial skin growth model at air-liquid interface.
- Pros and cons of standard cell culture vs. porous membrane cell culture
- Wide range of applications for Cell Culture Inserts
- Construction of artificial skin models at air-liquid interface on Cell Culture Insert
- Precise positioning of Cell Culture Inserts in the wells of the Carrier Plate
Presenter: Bob Scott, Application Specialist, Thermo Fisher Scientific
Optimizing results in your downstream imaging applications
Get the clear picture on your imaging applications workflow – from culturing and seeding your cells to image acquisition and data analysis - and how you can significantly improve your imaging results downstream by merely choosing the right culture vessel. In order to highlight the critical importance of using the right culture surface and format, we will present a case study of optimized neuron growth in chamber slides, in which we examine the correlation between neuron cell adhesion and multiple chamber slide surface modifications.
- Optimizing your image application workflow
- Key considerations when selecting a culture device
- Surface solutions for downstream imaging success
- Neuron culture and chamber slide case study
Presenter: Stephanie Carter, Application Specialist, Thermo Fisher Scientific
Eliminate the threat: Cleaning and Contamination in your Biological Safety Cabinet.
Contamination is the ever present threat to good science and your hard work. This webinar provides important points that will help you work with your biological safety cabinet more effectively to prevent contamination in your cell culture lab.
- The two types of contamination and steps to address each type
- Ways to safely clean different parts of your cabinet
- Dealing with special cases: spills, power failures, alarms, and decontaminations
Presenter: David S. Phillips, Technical Applications Specialist Laminar Flow, Thermo Fisher Scientific
Harnessing New Dimensions in Your Research: Growing 3D Cultures Consistently and Repeatedly.
Keeping pace with innovation can be exciting (but a bit tedious at times). We want to simplify things for you, offering new, indispensable tools that can be easily inserted into your cell culture process—saving you precious time and improving consistency of your research.
During this webinar you will learn about a novel surface technology that enables consistent, repeatable growth of spheroid cultures across many different cell types, including transformed cell lines and pluripotent stem cells.
- Closely simulate tumor progression in vitro
- Assess efficacy of anti-cancer agents
- Generate embryoid bodies for germ layer differentiation
- Form 3D cellular structures for many cell types
Presenter: Laura E. Jensen, Ph.D., Thermo Fisher Scientific
Get a Handle on Liquid Handling. Increase pipetting efficiency for reproducible results.
One of the most common tools in any cell culture laboratory is a pipette. You turn to it time and time again, and like most tools, finding the right fit for your lab is important to enable accurate and precise transferring of samples. During this webinar, we will review common requirements for cell culture applications, and key considerations to provide worry-free pipetting for confidence in your results.
- The role of pipetting systems in cell culture
- Identify the proper fit for your lab
- Reduce tedious steps to improve pipetting consistency
- Tips for working with complex protocols
Presenter: Paula Heimler, Field Product Manager, Liquid Transfer, Thermo Fisher Scientific
Nurture your incubator; nurture your cells. Proper Care and “Feeding” of Your Cell Culture Incubator
You trust your work—your cells--to your CO2 incubator. So if a problem occurs, it can be catastrophic for your research, setting you back weeks or months. This webinar provides important points that will help you avoid common mistakes, reduce contamination, and extend the life of your incubator.
- Proper positioning and installation of your cell culture incubator
- Best procedure for cleaning and disinfection, including tips for best results with automated high heat sterilization cycles
- Simple maintenance for best results, including when to calibrate, and why distilled water is important
Presenter: Mary Kay Bates, M.S., Global Cell Culture Specialist, Thermo Fisher Scientific
No more separation anxiety! A research case study: Gentle, non-enzymatic detachment of sensitive cells
What if you could gently detach even the most sensitive cells without enzymes or scraping, all while maintaining the effectiveness of your process, increasing the ease and speed of detachment and preserving cell surface proteins? Please join research scientist and esteemed colleague, Marten B. Maeß from Friedrich Schiller University – Jena, Germany – as he presents his research showing the application of an innovative surface to enable transfection and non-invasive detachment of macrophages. This research case study can be applied to diverse applications and cell types, offering insights into viable alternatives to your cell detachment methods.
- A research overview: Approaches to transfection and transfection of human macrophages
- Optimizing macrophage transfection protocol
- Detachment of cells by temperature reduction
- Consistent transfection efficiency and preserved functionality of the transfected cells
Presenter: Marten B. Maeß Research Scientist Friedrich Schiller University Recorded: April 17, 2014
The seven keys to safeguarding your cryopreserved cells
Safeguarding your samples throughout the entire cryopreservation process can be tricky and even unproductive, thanks to protocols that unknowingly undermine your results. Understanding the seven keys to protecting your cryopreserved cells – from selecting the right storage temperatures and proper cooling methods to avoiding common safety hazards when using liquid nitrogen – can equip any lab with the knowledge necessary to ensure sample safety and optimal cryopreservation results. During this webinar, we will discuss each of The Seven Keys along with the benefits you can achieve as a result.
- The big picture: The cryopreservation process
- What you need to know about cryopreservation temperatures and cooling rates
- Demystifying the tube selection process and knowing the vital features for sample safety
- Avoiding critical risks when using liquid nitrogen
Presenter: Jason Xu, Ph.D. Recorded: March 20, 2014
Take your Samples for a Spin: Fundamentals of Cell Culture Centrifugation
Separating cells is a critical step in the dynamic process of cell culture. This webinar will examine the role of centrifugation in the cell culture workflow and explore the different types of centrifuges used to separate cells. We will look at centrifuge requirements and technical considerations for cell culture applications, including selecting the appropriate speed and g-force. State-of-the-art trends in centrifugation will also be discussed.
- The role of centrifugation in cell culture
- Centrifuge requirements for cell culture applications
- Selecting the appropriate speed and g-force for your cell type
- Trends in cell culture centrifugation
Presenter: Ileana Place, Ph.D. Recorded: February 20, 2014
Avoid the icebergs! Managing sample preparation for cryo storage
Determining the proper sample preparation technique for long-term cryopreservation storage is of paramount importance to all downstream work stemming from the affected samples. During this webinar, we will discuss what happens during the sample preparation process, key concepts to be familiar with and key questions to consider when making decisions about long-term storage sample preparation.
- Sample preparation in relation to long-term cryopreservation storage
- Different methodologies in sample prep for cryopreservation storage
- Criteria to account for an optimized sample prep experience
Presenter: Dr. Alex Esmon Recorded: January 30, 2014
Maintaining Integrity: Selecting the Right Storage Tube for Downstream Cell Culture
Ensuring storage integrity and traceability of cells is of particular importance for cell culture applications. This webinar will provide detail related to cell preservation and storage considerations, as well as criteria for selecting appropriate storage tubes to meet your downstream cell culture needs.
- Considerations for cell preservation methods
- Storage tube selection criteria and why these are important to cell culture applications
- Importance of maintaining sample integrity and traceability through the use of barcoded tubes and complimentary accessories
Presenter: Kiara Biagioni Recorded: December 12, 2013
Optimize your cell culture workflow with Thermo Scientific™ peristaltic pumps
Optimizing cell culture processes is often dependant on how the cell culture fluid and media are handled. The proper selection of fluid handling equipment – pumps – can help improve efficiency and production in bio-processing research and production.
- Application of pumping systems in the cell culture process
- Tips for preventing contamination in fluid handling systems
- Optimizing cell growth environments
- Improving filtration process through improved pumping systems
- Increasing efficiency of dispensing processes
Presenter: Gregg Johnson Recorded: October 24, 2013
Choosing the right cold storage for cell culture applications
Provides practical pointers on how to select the best cold storage and sample prep solutions, with an eye to pertinent criteria that can lead to an optimized cell culture experience.
- Cold storage touch points in the cell culture workflow
- Types of cold storage products: how and what differentiates them
- Brief discussion of performance characteristics
- Criteria to consider for an optimized experience
Presenter: Dr. Alex Esmon Recorded: July 18, 2013
Surface Selection: Achieving optimal growth and harvesting of your cells
In this webinar, we give an overview of established and novel technologies used to modify cultureware surfaces for optimally growing adherent as well as non-adherent mammalian cells. We characterize the different Thermo Scientific™ Nunc™ surfaces by their adhesion properties and provide examples of how best to select the best culture surface for your application. We also present alternative methods to help improve cell harvesting.
- Different technologies for surface modification improving cell adhesion
- Overview and characterization of Nunc cell culture surfaces arranged by adhesion properties
- Examples of how to select the right surface for an application
- Alternative methods for advanced cell harvesting
Presenter: Dr. Thomas Stelzer Recorded: May 16, 2013
Protect You and Your Science - with biological safety cabinets
Effective use of the Class II biological safety cabinet (BSC) protects you and your important work. Explore how BSCs use effective air filtration and controlled airflow to prevent contamination from passing into the BSC.
- How BSC filters are different from most air filters
- The three airflows of the BSC; inflow, downflow and exhaust
- Selecting among the common types of Class II BSCs
- How to get the most out of any BSC
- Five myths of BSC operation
Presenter: Dave Phillips Recorded: April 11, 2013
Choosing the ideal lab water system for your cell culture applications
- How water impurities affect your cell culture experiments
- Choosing the right water purification system for your applications
- Latest trends in lab water systems
- Proper system maintenance
Presenter: Julie Akana Recorded: March 14, 2013
Take your Cell Based Assays to the Edge
- Reducing Edge Effects for Full Plate Results
- Increasing Assay Performance
- Improving Long-Term Cell Based Kinetics
Presenter: Sarah Verna, Christian Oberdanner Recorded: January 31, 2013
Culture Your Cells Using Less Oxygen for Biologically Relevant Results
- History and advances in understanding oxygen concentration
- Mechanism of cellular oxygen sensing and response
- Effects of low oxygen (hypoxia) on cultured cells
Presenter: Mary Kay Bates Recorded: December 6, 2012
The Last Line of Defense: Cell Culture Contamination and Using Filtration as a Final Preventative Measure
- Understanding and protecting against biological contamination
- Types, detection, sources and reducing risk
- How to choose the right filter
- Types of filter membranes and how they work
Presenter: Dennis Gorlick Recorded: November 8, 2012
Cell Culture Contamination: What You Don't Know Can Cost You
- Varied types of contamination and the extent of the problem
- Reducing contamination in your laboratory
- How to evaluate CO2 incubator design innovations for contamination prevention
Presenter: Mary Kay Bates Recorded: October 11, 2012