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Evolution™ 300 UV-Vis Spectrophotometer

The Thermo Scientific™ Evolution 300 is ideal for life science, pharmaceutical and QA/QC applications. A wide selection of accessories and a low cost of ownership make it a versatile and affordable addition to the lab. The powerful Xenon lamp-based platform and variable bandwidth make the Evolution 300 ideal for life science, pharmaceutical and QA/QC applications. A selection of VISION software packages expand instrument capability and Smart Accessories make your analyses easier to perform. The walk-up convenience of the Xenon lamp saves valuable time and increases your lab's productivity.

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10300101
Evolution 300 Security UV-Vis Spectrophotometer
Full specifications
Accuracy ±0.004A at 1A, ±0.004A at 2A, ±0.006A at 3AÅ
Accuracy (Photometric) ±0.004A at 1A, ± 0.004A at 2A, ± 0.006A at 3A
Baseline Flatness ±0.0015A (200-800nm), 2.0nm SBW, smoothed
Beam Geometry Dual-Beam; Quartz Coated
Certifications/Compliance 21 CFR Part 11 and audit-proof IQ/OQ/PQ documentation. ISO 9001:2000
Connections RS-232
Depth (English) 21 in.
Depth (Metric) 53 cm
Description Evolution 300 Security UV-Vis Spectrophotometer
Detector Type Dual Matched Silicon Photodiodes
Drift <0.0005Abs/hr, 500 nm, 2.0 nm SBW, 2hr warm-up
Electrical Requirements 100/240 V 50/60 Hz
Height (English) 15 in.
Height (Metric) 38 cm
Hertz 50/60 Hz
Includes VisionSecurity Software, User Guide and USB Cable
Item Description 300 PC w/VisionSecurity Software
Interface Computer Control
Lamp Xenon Flash Lamp
Typical lifetime: >5 years, longer if not using live signal
Warrenty period: 3 year source replacement warrenty
Min. Data Interval 0.5 nm
Monochromator Modified Ebert
Noise

Photometric:

0A: <0.00018 A

1A: <0.00022 A

2A: <0.00050 A

500 nm, 2.0 nm SBW, RMS

Optical Design

Modified Ebert

Double beam with sample and reference cuvette/accessory positions

Pathlength (Metric) Up to 100 mm cuvettes
Pharmacopoeia Compliance Testing

Resolution (Toluene in Hexane): Peak/Trough Ratio >2.0

Photometric Accuracy (60mg/L K2Cr2O7): <±0.01 A relative to calibrated sealed standard

Stray Light: <0.13 %T at 198 nm with KCl per EP

220 nm: <0.01%T NaI (behaves identically to KI for USP)

Wavelength Accuracy: ±0.20 nm (546.11 nm Hg emission line), ±0.30 nm 190–900 nm

Wavelength Repeatability: Peak separation of repetitive scanning of Hg line source <0.10 nm

Photometric Accuracy Including Standard Tolerance

1A: ±0.008 A

2A: ±0.010 A

3A: ±0.018 A

Photometric Display ±6 A
Photometric Range >4 A
Photometric Readout Absorbance, % Transmittance, % Reflectance, Concentration
Photometric Repeatability 1A: ±0.0025 A
Range (Photometric) >4A
Resolution (Toluene/Hexane) Peak/Trough >2.0 at 0.5nm SBW
Scan Ordinate Modes Absorbance, % Transmittance, % Reflectance, Concentration,
1st-4th Derivative
Scan Speed 1 - 3,800 nm/min
Spectral Bandwidth Variable 0.5; 1.0; 1.5; 2.0; 4.0 nm
Stray Light

198 nm: 2.9 A KCl

220 nm: 4.2 A NaI

340 nm: 4.3 A NaNO2

System Requirements At least Windows XP
Voltage 100/240 V
Warranty 3 years on Xenon Lamp
Wavelength Accuracy ±0.20 nm (546.11 nm Hg emission line); ±0.3 nm for 190 - 900 nm
Wavelength Data Interval 10, 5, 2, 1, 0.5, 0.2, 0.1, 0.05 nm
Wavelength Range 190 to 1100nm
Wavelength Repeatability Standard deviation of 10 measurements <0.05 nm
Wavelength Scan Speed 3800, 2400, 1200, 600, 240, 120, 60, 30, 10, 5, 1 nm/min Intelliscan
Weight (English) 48.4 lb.
Weight (Metric) 22 kg
Width (English) 24 in.
Width (Metric) 61 cm

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    10300201
    Evolution 300 PC UV-Vis Spectrophotometer
    Full specifications
    Accuracy ±0.004A at 1A, ±0.004A at 2A, ±0.006A at 3AÅ
    Accuracy (Photometric) ±0.004A at 1A, ± 0.004A at 2A, ± 0.006A at 3A
    Baseline Flatness ±0.0015A (200-800nm), 2.0nm SBW, smoothed
    Beam Geometry Dual-Beam; Quartz Coated
    Certifications/Compliance 21 CFR Part 11 and audit-proof IQ/OQ/PQ documentation. ISO 9001:2000
    Connections RS-232
    Depth (English) 21 in.
    Depth (Metric) 53cm
    Description Evolution 300 PC UV-Vis Spectrophotometer
    Detector Type Dual Matched Silicon Photodiodes
    Drift <0.0005Abs/hr, 500 nm, 2.0 nm SBW, 2hr warm-up
    Electrical Requirements 100/240V 50/60Hz
    Height (English) 15 in.
    Height (Metric) 38cm
    Hertz 50/60Hz
    Includes VisionSecurity Software, User Guide and USB Cable
    Item Description 300 PC w/VisionPro Software
    Interface Computer Control
    Lamp Xenon Flash Lamp
    Typical lifetime: >5 years, longer if not using live signal
    Warrenty period: 3 year source replacement warrenty
    Min. Data Interval 0.5nm
    Monochromator Modified Ebert
    Noise

    Photometric:

    0A: <0.00018 A

    1A: <0.00022 A

    2A: <0.00050 A

    500 nm, 2.0 nm SBW, RMS

    Optical Design

    Modified Ebert

    Double beam with sample and reference cuvette/accessory positions

    Pathlength (Metric) Up to 100mm cuvettes
    Pharmacopoeia Compliance Testing

    Resolution (Toluene in Hexane): Peak/Trough Ratio >2.0

    Photometric Accuracy (60mg/L K2Cr2O7): <±0.01 A relative to calibrated sealed standard

    Stray Light: <0.13 %T at 198 nm with KCl per EP

    220 nm: <0.01%T NaI (behaves identically to KI for USP)

    Wavelength Accuracy: ±0.20 nm (546.11 nm Hg emission line), ±0.30 nm 190–900 nm

    Wavelength Repeatability: Peak separation of repetitive scanning of Hg line source <0.10 nm

    Photometric Accuracy Including Standard Tolerance

    1A: ±0.008 A

    2A: ±0.010 A

    3A: ±0.018 A

    Photometric Display ±6 A
    Photometric Range >4 A
    Photometric Readout Absorbance, % Transmittance, % Reflectance, Concentration
    Photometric Repeatability 1A: ±0.0025 A
    Range (Photometric) >4A
    Resolution (Toluene/Hexane) Peak/Trough >2.0 at 0.5nm SBW
    Scan Ordinate Modes Absorbance, % Transmittance, % Reflectance, Concentration,
    1st-4th Derivative
    Scan Speed 1 to 3800nm/min
    Spectral Bandwidth Variable 0.5; 1.0; 1.5; 2.0; 4.0 nm
    Stray Light

    198 nm: 2.9 A KCl

    220 nm: 4.2 A NaI

    340 nm: 4.3 A NaNO2

    System Requirements At least Windows XP
    Voltage 100/240V
    Warranty 3 years on Xenon Lamp
    Wavelength Accuracy ±0.20nm (546.11nm Hg emission line); ±0.3nm for 190 to 900nm
    Wavelength Data Interval 10, 5, 2, 1, 0.5, 0.2, 0.1, 0.05 nm
    Wavelength Range 190 to 1100nm
    Wavelength Repeatability Standard deviation of 10 measurements <0.05 nm
    Wavelength Scan Speed 3800, 2400, 1200, 600, 240, 120, 60, 30, 10, 5, 1 nm/min Intelliscan
    Weight (English) 48.4 lb.
    Weight (Metric) 22kg
    Width (English) 24 in.
    Width (Metric) 61cm

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      10300501
      Evolution 300 PC UV-Vis Spectrophotometer, No Software
      Full specifications
      Accuracy ±0.004A at 1A, ±0.004A at 2A, ±0.006A at 3AÅ
      Accuracy (Photometric) ±0.004A at 1A, ± 0.004A at 2A, ± 0.006A at 3A
      Baseline Flatness ±0.0015A (200-800nm), 2.0nm SBW, smoothed
      Beam Geometry Dual-Beam; Quartz Coated
      Certifications/Compliance 21 CFR Part 11 and audit-proof IQ/OQ/PQ documentation. ISO 9001:2000
      Connections RS-232
      Depth (English) 21 in.
      Depth (Metric) 53cm
      Description Evolution 300 PC UV-Vis Spectrophotometer, No Software
      Detector Type Dual Matched Silicon Photodiodes
      Drift <0.0005Abs/hr, 500 nm, 2.0 nm SBW, 2hr warm-up
      Electrical Requirements 100/240V 50/60Hz
      Height (English) 15 in.
      Height (Metric) 38cm
      Hertz 50/60Hz
      Includes VisionSecurity Software, User Guide and USB Cable
      Item Description 300 PC, No Software
      Interface Computer Control
      Lamp Xenon Flash Lamp
      Typical lifetime: >5 years, longer if not using live signal
      Warrenty period: 3 year source replacement warrenty
      Min. Data Interval 0.5nm
      Monochromator Modified Ebert
      Noise

      Photometric:

      0A: <0.00018 A

      1A: <0.00022 A

      2A: <0.00050 A

      500 nm, 2.0 nm SBW, RMS

      Optical Design

      Modified Ebert

      Double beam with sample and reference cuvette/accessory positions

      Pathlength (Metric) Up to 100mm cuvettes
      Pharmacopoeia Compliance Testing

      Resolution (Toluene in Hexane): Peak/Trough Ratio >2.0

      Photometric Accuracy (60mg/L K2Cr2O7): <±0.01 A relative to calibrated sealed standard

      Stray Light: <0.13 %T at 198 nm with KCl per EP

      220 nm: <0.01%T NaI (behaves identically to KI for USP)

      Wavelength Accuracy: ±0.20 nm (546.11 nm Hg emission line), ±0.30 nm 190–900 nm

      Wavelength Repeatability: Peak separation of repetitive scanning of Hg line source <0.10 nm

      Photometric Accuracy Including Standard Tolerance

      1A: ±0.008 A

      2A: ±0.010 A

      3A: ±0.018 A

      Photometric Display ±6 A
      Photometric Range >4 A
      Photometric Readout Absorbance, % Transmittance, % Reflectance, Concentration
      Photometric Repeatability 1A: ±0.0025 A
      Range (Photometric) >4A
      Resolution (Toluene/Hexane) Peak/Trough >2.0 at 0.5nm SBW
      Scan Ordinate Modes Absorbance, % Transmittance, % Reflectance, Concentration,
      1st-4th Derivative
      Scan Speed 1 to 3800nm/min
      Spectral Bandwidth Variable 0.5; 1.0; 1.5; 2.0; 4.0 nm
      Stray Light

      198 nm: 2.9 A KCl

      220 nm: 4.2 A NaI

      340 nm: 4.3 A NaNO2

      System Requirements At least Windows XP
      Voltage 100/240V
      Warranty 3 years on Xenon Lamp
      Wavelength Accuracy ±0.20nm (546.11nm Hg emission line); ±0.3nm for 190-900nm
      Wavelength Data Interval 10, 5, 2, 1, 0.5, 0.2, 0.1, 0.05 nm
      Wavelength Range 190 to 1100nm
      Wavelength Repeatability Standard deviation of 10 measurements <0.05 nm
      Wavelength Scan Speed 3800, 2400, 1200, 600, 240, 120, 60, 30, 10, 5, 1 nm/min Intelliscan
      Weight (English) 48.4 lb.
      Weight (Metric) 22kg
      Width (English) 24 in.
      Width (Metric) 61cm

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        Product Description

        The powerful Xenon lamp-based platform and variable bandwidth make the Evolution 300 ideal for life science, pharmaceutical and QA/QC applications. A selection of VISION software packages expand instrument capability and Smart Accessories make your analyses easier to perform. The walk-up convenience of the Xenon lamp saves valuable time and increases your lab's productivity.


        Xenon Lamp

        • Provides excellent performance over the full wavelength range
        • Ready to use without instrument warmup for fast readings
        • Activates only when taking a measurement; extended lamp life lowers cost of instrument ownership
        • 3-year warranty


        Key Features

        • 5 variable spectral bandwidth settings for matching customer assays to instrument
        • Acquisition rate: 50 data points per second
        • Longlife Xenon lamp


        Optional Smart Accessories For Any Application

        • Full line available for Life Sciences and QA/QC
        • Install or remove without restarting the instrument
        • Built-in software instantly identifies and configures to accommodate accessory
        • Each accessory has a unique serial number that is recorded for comprehensive event logs and audit trail capabilities
        • Automated performance verification with CVC accessory
        • Mercury lamp accessory is available for testing and defining wavelength accuracy


        PC Control

        • Enables data collection and distribution to third party applications for advanced processing and archiving
        • Uses powerful VISION software suite to control instrument
        • Collects, stores, recalls and analyzes data

         

        Ordering Information:

        All accessories are sold separately. Available software packages include VISIONliteMaterialsCalc (for material analysis), VISIONpro (standard UV-VIS), VISIONlife (Life Science), VISIONsecurity (regulated labs), VISIONlite ColorCalc Basic (XYZ, Lab, CIE), and VISIONlite ColorCalc Advanced (>40DAB/EP color check filters, Pt-Co/Alpha color, Gardner and Iodine color, wine analysis). Other accessories include cell changers, cuvet and test holders, calibration carousels, sippers, fiber optic probes, small volume sampling, and accessories for reflectance and rapid mixing.


        Recommended for:

        • General Spectroscopy
        • High-resolution UV-Visible spectrscopy
        • Temperature-based investigations

        Life Science

        • Nucleic acid assays
        • Protein concentration assays
        • Thermal denaturation/renaturation
        • Kinetics

        QA/QC

        • Quantitative analysis
        • Finished product analysis
        • Measurements in the regulated laboratory

        Pharmaceuctical

        • General Research
        • QA/QC
        • Tablet dissolution


        Note:

        To use the Evolution 300 UV-Vis Spectrophotometer in a regulated or quality control environment, we offer a wide selection of compliance products and services to reduce implementation time and assure system performance.

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        Spectrophotometer Guide

        Compare the SPECTRONIC, GENESYS and Evolution spectrophotometers. Watch videos, read applications and find dealers near you.

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