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Varioskan™ Flash Multimode Reader

Run demanding research assays with the utmost flexibility using the Thermo Scientific™ Varioskan™ Flash Multimode Reader. This versatile instrument can be used across a wide variety of applications. It offers unlimited wavelength selection, up to three onboard dispensers and excellent optical performance. The Varioskan Flash Multimode Reader has passed the DLReady™ validation at Promega Corporation™ and is certified as DLReady. Additionally, the reader has been granted the LanthaScreen™ Certified designation by Invitrogen™, and is validated for Thermo Scientific Pierce™ Luciferase Assays.

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Product Description

Varioskan Flash spectral scanning multimode reader includes fluorescence intensity, time-resolved fluorescence (TRF), photometric, and optional luminometric detection technologies. It is designed for optimization and analysis of various assays, such as binding assays, ADMETox, molecular biology assays, enzyme kinetic studies, ion-channel and cell signalling assays.

Features:
  • Unlimited wavelength selection for spectral analysis and measurement at any single wavelength
  • Easy measurement setup with automated internal functions ensuring maximal sensitivity and full dynamic range for every assay
  • Onboard dispensers for exact follow-up of kinetic reactions
  • Increased assay throughput—reads up to 1536-well plates and can easily be integrated with automated systems
  • High performance incubator for temperature critical assays
  • Easy assay setup, flexible data handling and convenient report formatting with Thermo Scientific™ SkanIt™ Software

Compatible with:

The Varioskan Flash is compatible with the Thermo Scientific™ μDrop plate.

Recommended for:

Apoptosis assays; Ca2+ flux assays; Cell proliferation; Cellular assays; Cytotoxicity and ADMETox; Direct DNA, RNA and protein quantitation; ELISA/FIA/TRF-ELISA assays; Enzyme kinetic studies; Europium assays; FRET assays; TR-FRET assays; BRET assays; GPCR assays; Ion channel assays; Kinase assays; Multilabel assays; Reporter gene assays; Signal transduction; Tryptophan and tyrosine UV fluorescence

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Determination of Protein Unfolding using UV-fluorometry

Measurement parameters for protein unfolding assay with Thermo Scientific Varioskan Flash spectral scanning multitechnology reader were optimized using lysozyme and BSA as example proteins.

 
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Showing 4 4 of 4 Products
Catalog #
Description
Quantity
5250030
Varioskan Flash fluorescence intensity (top reading), photometric and time-resolved fluorescence measurement capabilities. (For luminometric reading capability Varioskan LumiSens option is required)
Full specifications
Description Varioskan Flash fluorescence intensity (top reading), photometric and time-resolved fluorescence measurement capabilities. (For luminometric reading capability Varioskan LumiSens option is required)
Measurement Type Fluorescence intensity, time-resolved fluorescence, photometry and optional luminometry (Varioskan LumiSense option required), all with spectral scanning
Plate Type Six 1536-Well Plates; Photometry: Six 384-Well Plates
Light Source Xenon Flash lamp
Wavelength Selection Fluorometry: Double excitation and double emission monochromators
Photometry: Double monochromators
Wavelength Range
Fluorometry: Excitation: 200 to 1000nm, Emission: 270 to 840nm
Photometry: 200 to 1000nm
Photometric Linearity 0 to 4 Abs, (96-well plate) at 450nm, ±2%; 0 to 3 Abs (384-well plate) at 450nm, ±2%
Accuracy (Photometric)
±2% or 0.003 Abs, whichever is greater, at 200 to 399nm (0 to 2 Abs)
±1% or 0.003 Abs, whichever is greater, at 400 to 1000nm (0 to 3 Abs)
Precision SD <0.001 Abs or CV <0.5%, whichever is greater, at 450nm (0 to 3 Abs)
Sensitivity Fluorescence intensity, top reading: <0.4 fmol fluorescein/well, 384-well plate
Time-resolved fluorescence, top reading: <120 amol Europium/well, 384-well plate
Dynamic Range Fluorescence intensity, top reading: >6 decades
Time-resolved fluorescence, top reading: >6 decades
No. of Dispensers Up to 3 optional dispensers
Incubator Temperature Ambient +4° to 45°C
Shaking Orbital
Measurement Speed 96-well plate in 15 seconds, 384-well plate in 45 seconds, and 1536-well plate in 135 seconds (minimum kinetic interval time from A1 back to A1
Depth (Metric) 58cm
Width (Metric 54cm
Width (English) 21.3 in.
Height (Metric) 50cm
Height (English) 19.7 in.
Weight (Metric) 55 to 67kg, depending on the configuration
Weight (English) 121 to 148 lb., depending on the configuration

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    5250040
    Varioskan Flash fluorescence intensity (top and bottom reading), photometric and time-resolved fluorescence measurement capabilities. (For luminometric reading capability Varioskan LumiSens option is required)
    Full specifications
    Description Varioskan Flash fluorescence intensity (top and bottom reading), photometric and time-resolved fluorescence measurement capabilities. (For luminometric reading capability Varioskan LumiSens option is required)
    Measurement Type Fluorescence intensity, time-resolved fluorescence, photometry and optional luminometry (Varioskan LumiSense option required), all with spectral scanning
    Plate Type Six 1536-Well Plates; Photometry: Six 384-Well Plates
    Light Source Xenon Flash lamp
    Wavelength Selection Fluorometry: Double excitation and double emission monochromators
    Photometry: Double monochromators
    Wavelength Range
    Fluorometry: Excitation: 200 to 1000nm, Emission: 270 to 840nm
    Photometry: 200 to 1000nm
    Photometric Linearity 0 to 4 Abs, (96-well plate) at 450nm, ±2%; 0 to 3 Abs (384-well plate) at 450nm, ±2%
    Accuracy (Photometric) ±2% or 0.003 Abs, whichever is greater, at 200 to 399nm (0 to 2 Abs) ±1% or 0.003 Abs, whichever is greater, at 400 to 1000nm (0 to 3 Abs)
    Precision SD <0.001 Abs or CV <0.5%, whichever is greater, at 450nm (0 to 3 Abs)
    Sensitivity Fluorescence intensity, top reading: <0.4 fmol fluorescein/well, 384-well plate
    Fluorescence intensity, bottom reading: <0.4 fmol fluorescein/well, 384-well plate
    Time-resolved fluorescence, top reading: <120 amol Europium/well, 38
    Dynamic Range Fluorescence intensity, top reading: >6 decades; bottom reading: >5.5 decades
    Time-resolved fluorescence, top reading: >6 decades
    No. of Dispensers Up to 3 optional dispensers
    Incubator Temperature Ambient +4° to 45°C
    Shaking Orbital
    Measurement Speed 96-well plate in 15 seconds, 384-well plate in 45 seconds, and 1536-well plate in 135 seconds (minimum kinetic interval time from A1 back to A1
    Depth (Metric) 58cm
    Width (Metric 54cm
    Width (English) 21.3 in.
    Height (Metric) 50cm
    Height (English) 19.7 in.
    Weight (Metric) 55 to 67kg, depending on the configuration
    Weight (English) 121 to 148 lb., depending on the configuration

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      5250500
      Varioskan LumiSens option
      Full specifications
      Description Varioskan LumiSens option
      Plate Type Six 1536-Well Plates
      Wavelength Selection Luminometry: All wavelengths, filters and double monochromators
      Sensitivity Luminometry: <7 amol ATP/well, flash ATP reaction, 384-well plate
      Dynamic Range Luminometry: >7 decades

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        5250510
        Dispenser option, with 1mL syringe, factory fitted
        Full specifications
        Description Dispenser option, with 1mL syringe, factory fitted
        Plate Type Six 384-Well Plates
        No. of Dispensers One dispenser, factory fitted (up to 3 optional dispensers can be installed to Varioskan Flash)

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