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Varioskan™ Flash Multimode Reader

Run demanding research assays with the utmost flexibility using the Thermo Scientific™ Varioskan™ Flash Multimode Reader.  The Varioskan Flash Multimode Reader has passed the DLReady™ validation at Promega Corporation™ and is certified as DLReady. Additionally, the reader has been granted the LanthaScreen™ Certified designation by Invitrogen™, and is validated for Thermo Scientific Pierce™ Luciferase Assays.

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Catalog #
Includes
Quantity
5250030
Varioskan Flash with top reading
Full specifications
Light Source Xenon Flash
Wavelength Selection Fluorometry: Double excitation and double emission monochromators; Luminometry: All wavelengths, filters and double monochromators; Photometry: Double monochromators
Emission Wavelength Range 270 to 840nm
Precision SD <0.001 Abs or CV <0.5%, whichever is greater, at 450nm (0 to 3 Abs)
Sensitivity Luminometry: <7 amol ATP/well, flash ATP reaction, 384-well plate; Fluorescence intensity, top reading: <0.4 fmol fluorescein/well, 384-well plate; Fluorescence intensity, bottom; Time-resolved fluorescence, top reading: <120 amol Europium/well, 384-well
Dynamic Range Luminometry: >7 decades; Fluorometry: Fluorescence intensity, top reading: >6 decades; bottom reading: >5.5 decades; Time-resolved fluorescence, top reading: >6 decades
Incubator Temperature Ambient +4 to 45°C
Shaking Orbital
Spectral Scanning Speed <2s/well, 400 to 500nm, 1 flash, 2nm steps
Depth (Metric) 58cm
Depth (English) 22.8 in.
Width (Metric) 54cm
Width (English) 21.3 in.
Height (Metric) 50cm
Height (English) 19.7 in.
Weight (Metric) 55 to 67kg
Weight (English) 121 - 148 lb.
Accepts 6 - 1536 Well Plates; Photometry: 6 -384 Well Plates
Accuracy (Photometric) SD <0.001 Abs or CV <0.5%, whichever is greater, at 450nm (0 to 3 Abs)
Count Up to 3
Compatibility µDrop plate
Excitation Wavelength Range Fluorometry: 200 to 1000nm; Luminometry: 360 to 670nm
Includes Varioskan Flash with top reading
Item Description Varioskan Flash with top reading
Linearity 0 to 4 Abs, (96-well plate) at 450nm, ±2%; 0 to 3 Abs (384-well plate) at 450nm, ±2%
Measurement Speed 96-well plate in 15 seconds, 384-well plate in 45 seconds, and 1536-well plate in 135 seconds (minimum kinetic interval time from A1 back to A1
Measurement Type Fluorescence intensity, time-resolved fluorescence, photometry and optional luminometry, all with spectral scanning

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    5250040
    Varioskan Flash with top, bottom reading
    Full specifications
    Light Source Xenon Flash
    Wavelength Selection Fluorometry: Double excitation and double emission monochromators; Luminometry: All wavelengths, filters and double monochromators; Photometry: Double monochromators
    Emission Wavelength Range 270 to 840nm
    Precision SD <0.001 Abs or CV <0.5%, whichever is greater, at 450nm (0 to 3 Abs)
    Sensitivity Luminometry: <7 amol ATP/well, flash ATP reaction, 384-well plate; Fluorescence intensity, top reading: <0.4 fmol fluorescein/well, 384-well plate; Fluorescence intensity, bottom; Time-resolved fluorescence, top reading: <120 amol Europium/well, 384-well
    Dynamic Range Luminometry: >7 decades; Fluorometry: Fluorescence intensity, top reading: >6 decades; bottom reading: >5.5 decades; Time-resolved fluorescence, top reading: >6 decades
    Incubator Temperature Ambient +4 to 45°C
    Shaking Orbital
    Spectral Scanning Speed <2s/well, 400 to 500nm, 1 flash, 2nm steps
    Depth (Metric) 58cm
    Depth (English) 22.8 in.
    Width (Metric) 54cm
    Width (English) 21.3 in.
    Height (Metric) 50cm
    Height (English) 19.7 in.
    Weight (Metric) 55 to 67kg
    Weight (English) 121 - 148 lb.
    Accepts 6 - 1536 Well Plates; Photometry: 6 -384 Well Plates
    Accuracy (Photometric) SD <0.001 Abs or CV <0.5%, whichever is greater, at 450nm (0 to 3 Abs)
    Count Up to 3
    Compatibility µDrop plate
    Excitation Wavelength Range Fluorometry: 200 to 1000nm; Luminometry: 360 to 670nm
    Includes Varioskan Flash with top, bottom reading
    Item Description Varioskan Flash with top, bottom reading
    Linearity 0 to 4 Abs, (96-well plate) at 450nm, ±2%; 0 to 3 Abs (384-well plate) at 450nm, ±2%
    Measurement Speed 96-well plate in 15 seconds, 384-well plate in 45 seconds, and 1536-well plate in 135 seconds (minimum kinetic interval time from A1 back to A1
    Measurement Type Fluorescence intensity, time-resolved fluorescence, photometry and optional luminometry, all with spectral scanning

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      5250500
      Varioskan LumiSens option
      Full specifications
      Light Source Xenon Flash
      Wavelength Selection Fluorometry: Double excitation and double emission monochromators; Luminometry: All wavelengths, filters and double monochromators; Photometry: Double monochromators
      Emission Wavelength Range 270 to 840nm
      Precision SD <0.001 Abs or CV <0.5%, whichever is greater, at 450nm (0 to 3 Abs)
      Sensitivity Luminometry: <7 amol ATP/well, flash ATP reaction, 384-well plate; Fluorescence intensity, top reading: <0.4 fmol fluorescein/well, 384-well plate; Fluorescence intensity, bottom; Time-resolved fluorescence, top reading: <120 amol Europium/well, 384-well
      Dynamic Range Luminometry: >7 decades; Fluorometry: Fluorescence intensity, top reading: >6 decades; bottom reading: >5.5 decades; Time-resolved fluorescence, top reading: >6 decades
      Incubator Temperature Ambient +4 to 45°C
      Shaking Orbital
      Spectral Scanning Speed <2s/well, 400 to 500nm, 1 flash, 2nm steps
      Depth (Metric) 58cm
      Depth (English) 22.8 in.
      Width (Metric) 54cm
      Width (English) 21.3 in.
      Height (Metric) 50cm
      Height (English) 19.7 in.
      Weight (Metric) 55 to 67kg
      Weight (English) 121 - 148 lb.
      Accepts 6 - 1536 Well Plates; Photometry: 6 -384 Well Plates
      Accuracy (Photometric) SD <0.001 Abs or CV <0.5%, whichever is greater, at 450nm (0 to 3 Abs)
      Count Up to 3
      Compatibility µDrop plate
      Excitation Wavelength Range Fluorometry: 200 to 1000nm; Luminometry: 360 to 670nm
      Includes Varioskan LumiSens option
      Item Description Varioskan LumiSens option
      Linearity 0 to 4 Abs, (96-well plate) at 450nm, ±2%; 0 to 3 Abs (384-well plate) at 450nm, ±2%
      Measurement Speed 96-well plate in 15 seconds, 384-well plate in 45 seconds, and 1536-well plate in 135 seconds (minimum kinetic interval time from A1 back to A1
      Measurement Type Fluorescence intensity, time-resolved fluorescence, photometry and optional luminometry, all with spectral scanning

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        5250510
        Dispenser option, with 1mL syringe
        Full specifications
        Light Source Xenon Flash
        Wavelength Selection Fluorometry: Double excitation and double emission monochromators; Luminometry: All wavelengths, filters and double monochromators; Photometry: Double monochromators
        Emission Wavelength Range 270 to 840nm
        Precision SD <0.001 Abs or CV <0.5%, whichever is greater, at 450nm (0 to 3 Abs)
        Sensitivity Luminometry: <7 amol ATP/well, flash ATP reaction, 384-well plate; Fluorescence intensity, top reading: <0.4 fmol fluorescein/well, 384-well plate; Fluorescence intensity, bottom; Time-resolved fluorescence, top reading: <120 amol Europium/well, 384-well
        Dynamic Range Luminometry: >7 decades; Fluorometry: Fluorescence intensity, top reading: >6 decades; bottom reading: >5.5 decades; Time-resolved fluorescence, top reading: >6 decades
        Incubator Temperature Ambient +4 to 45°C
        Shaking Orbital
        Spectral Scanning Speed <2s/well, 400 to 500nm, 1 flash, 2nm steps
        Depth (Metric) 58cm
        Depth (English) 22.8 in.
        Width (Metric) 54cm
        Width (English) 21.3 in.
        Height (Metric) 50cm
        Height (English) 19.7 in.
        Weight (Metric) 55 to 67kg
        Weight (English) 121 - 148 lb.
        Accepts 6 - 1536 Well Plates; Photometry: 6 -384 Well Plates
        Accuracy (Photometric) SD <0.001 Abs or CV <0.5%, whichever is greater, at 450nm (0 to 3 Abs)
        Count Up to 3
        Compatibility µDrop plate
        Excitation Wavelength Range Fluorometry: 200 to 1000nm; Luminometry: 360 to 670nm
        Includes Dispenser option, with 1mL syringe
        Item Description Dispenser option, with 1mL syringe
        Linearity 0 to 4 Abs, (96-well plate) at 450nm, ±2%; 0 to 3 Abs (384-well plate) at 450nm, ±2%
        Measurement Speed 96-well plate in 15 seconds, 384-well plate in 45 seconds, and 1536-well plate in 135 seconds (minimum kinetic interval time from A1 back to A1
        Measurement Type Fluorescence intensity, time-resolved fluorescence, photometry and optional luminometry, all with spectral scanning

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          Product Description

          Varioskan Flash spectral scanning multimode reader includes fluorescence intensity, time-resolved fluorescence (TRF), photometric, and optional luminometric detection technologies. It is designed for optimization and analysis of various assays, such as binding assays, ADMETox, molecular biology assays, enzyme kinetic studies, ion-channel and cell signalling assays.

          Features:
          • Unlimited wavelength selection for spectral analysis and measurement at any single wavelength
          • Easy measurement setup with automated internal functions ensuring maximal sensitivity and full dynamic
          • Onboard dispensers for exact follow-up of kinetic reactions
          • Increased assay throughput—reads up to 1536-well plates and can easily be integrated with automated systems
          • High performance incubator for temperature critical assays
          • Easy assay setup, flexible data handling and convenient report formatting with Thermo Scientific™ SkanIt™ Software

          Compatible with:

          The Varioskan Flash is compatible with the new Thermo Scientific™ μDrop plate.

          Recommended for:

          Apoptosis assays; Ca2+ flux assays; Cell proliferation; Cellular assays; Cytotoxicity and ADMETox; Direct DNA, RNA and protein quantitation; ELISA/FIA/TRF-ELISA assays; Enzyme kinetic studies; Europium assays; FRET assays; TR-FRET assays; BRET assays; GPCR assays; Ion channel assays; Kinase assays; Multilabel assays; Reporter gene assays; Signal transduction; Tryptophan and tyrosine UV fluorescence

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          Determination of Protein Unfolding using UV-fluorometry

          Measurement parameters for protein unfolding assay with Thermo Scientific Varioskan Flash spectral scanning multitechnology reader were optimized using lysozyme and BSA as example proteins.

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