Cellular Imaging and Analysis

Advanced high-content technology for cellular imaging and analysis


What is High Content Screening and Analysis?

High Content Screening (HCS), also known as High Content Analysis (HCA), or quantitative cellular imaging, is an automated method that is used to identify a phenotype or substances that alter the phenotype of a cell in a desired method by integrated mapping, image processing, and visualization tools to extract data from each cell and cell population to determine a response.


How Does High-Content Technology Work?

High Content is a combination of three standard tools in the cell biology laboratory: Fluorescence and Transmitted light microscopes for high resolution imaging; Plate Reader Technology, which is designed around measuring fluorescent intensities of targets in high throughput formats; and Flow Cytometry, which focuses on data from each cell. High-content technology is designed to measure:

  • Spatial distribution of targets in cells
  • Individual cell and organelle morphology
  • Combined multiple measurements per cell
  • Multiple populations of cells isolating multiple phenotypes


Our Solution

As the inventor of High-Content technology, the Thermo Scientific™ High Content Instruments, Informatics, and Assays portfolio, along with superior technical expertise, delivers significant impact across life science research from oncology, neurobiology through to in vitro toxicology.

Featured videos



Learn How the Thermo Scientific CellInsight HCS Platform Can Further Cancer Research

HCA Q and A