Fluoroskan Ascent Microplate Fluorometer ­ Thermo Scientific

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Fluoroskan Ascent Microplate Fluorometer

Thermo Scientific Fluoroskan Ascent Microplate Fluorometer is a compact and robust instrument with excellent optical performance for a variety of research applications.
The Fluoroskan Ascent Microplate Fluorometer is ideal for life science research applications, such as fluorometric protein and enzyme studies, molecular interactions, nucleic acid quantification, reporter gene, fluorometric kinase, immuno and cell based assays.
 
 
 
 
Part Number Description   Quantity
5210470 Fluoroskan Ascent
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5210480 Fluoroskan Ascent with one dispenser
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5210482 Fluoroskan Ascent with two dispensers
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5210483 Fluoroskan Ascent with three dispensers
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Please Note: Availability may vary by country.  
 
Fast reading speed

  • Enables the most rapid throughput
  • Great for a wide variety of applications, such as cytotoxicity, ion channel and FRET

Onboard dispensers for exact follow-up of kinetic reactions

  • Equipped with up to three reagent dispensers for fast kinetic assays, such as Ca2+ flux
  • Supports simultaneous dispensing and reading, enabling monitoring of fast kinetic measurements from the very start of the reaction
  • Very low dead volume and the backflush capability allow conservation of expensive reagents

High sensitivity for both top and bottom reading

  • Fiberless direct illumination optics for both top and bottom reading
  • Ensures a high sensitivity, a wide dynamic range, and accurate and precise results for 1- to 384-well plates

Specially designed for automation

  • To increase assay throughput, the Fluoroskan Ascent can read up to 384-well plates
  • Easily integrated with automated systems
Includes: PC Software and filter pairs: Excitation: 355nm/Emission: 460nm, Excitation: 485nm/ Emission: 538nm

Warranty and Service Offering: 1 year

Recommended for: Ca2+ flux assays; Cell proliferation; Cytotoxicity; Multi-drug resistance; Cell adhesion; DNA quantitation; Reporter gene assays; Hybridization assays; Quantitation of PCR products; FRET assays; Molecular beacon assays; Immunoassays; Enzyme activity; Neonatalogy; Bacterial quantitation; Phagocytosis; Oligonucleotide assays; ADMEtox
Specifications
Excitation Wavelength Range (Fluorometry)320 to 700nm
Emission Wavelength Range (Fluorometry)360 to 800nm
Excitation Filters (Fluorometry)Up to eight filters in the excitation filter wheel. 355 and 485nm filters included as standard. Other filters available upon request.
Emission Filters (Fluorometry)Up to eight filters in the emission filter wheel. 460 and 538nm filters included as standard. Other filters available upon request.
Sensitivity (Fluorometry)2fmol fluorescein/well in a black 96-well plate
Dynamic Range (Fluorometry)>6 decades
No of dispensersUp to 3
Dispensing volume1 to 1000µL in 1µL increments
Dispensing Speed25 seconds, 96-well plate, 5µL/well
Plate Types1- to 384-well plates
Measurement Speed15 seconds, 96-well plate
Wavelength selectionFilters
Light SourceQuartz-halogen lamp
DetectorPhotomultiplier Tube
IncubatorFrom ambient +3°C to 45°C, at ambient 25°C
ShakingOrbital shaker
Computer InterfaceSerial RS-232C port
User InterfaceRequires, but does not include, a personal computer
Dimensions (HxWxD)13.4 x 16.5 x 16.5 in. (34 x 42 x 42cm)
WeightBasic unit: 21kg (46 lbs.); three optional dispensers add 3.5kg

Cat. No.Description
5210470Fluoroskan Ascent
5210480Fluoroskan Ascent with one dispenser
5210482Fluoroskan Ascent with two dispensers
5210483Fluoroskan Ascent with three dispensers
 
 
 
 
 
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