Appliskan Multimode Microplate Reader ­ Thermo Scientific

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Appliskan Multimode Microplate Reader

Thermo Scientific Appliskan Multimode Microplate Reader is a filter-based multitechnology microplate reader for photometric, luminometric and fluorometric research applications.
The Appliskan offers all detection technologies in one small-sized, compact and robust instrument for both current and future application needs in an ever-changing research environment. It features an onboard shaker, an incubator and up to two onboard dispensers and can work with 6- to 384-well plates.
 
 
 
 
Part Number Description   Quantity
5230000 Appliskan
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5230010 Appliskan with one dispenser
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5230020 Appliskan with two dispensers
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460SP400 First Dispenser Kit
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460SP420 Second Dispenser Kit
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Please Note: Availability may vary by country.  
 
  • All technologies included—fluorometry, luminometry, absorbance, TRF and FP
  • Excellent sensitivity with luminometric and Time-Resolved-Fluorescence applications
  • Small footprint fits into even the most crowded laboratories
  • Versatile Thermo Scientific SkanIt Software provides easy assay design, flexible data handling and convenient report formatting, even with challenging applications
  • Numerous plate formats from 6- to 384-well
  • Up to two dispensers for exact follow-up of kinetic reactions
  • Fluorometric wavelength range up to 820nm provides a high sensitivity and a wide linear dynamic range in all fluorometric applications, even with red-sensitive fluorochromes and demanding TR-FRET applications
Includes: SkanIt Software, Excitation 485nm and Emission 535nm filters. Other filters available upon request.

Warranty and Service Offering: 1 year

Recommended for: Cellular assays; Cytotoxicity and Cell proliferation assays; DNA quantitation; ELISA/FIA/LIA assays; Enzyme kinetic studies; Europium assays; FRET and BRET assays; GPCR assays; Ion channel assays; Kinase assays; Multilabel assays; Phagocytosis; Protein assays; Reporter gene assays; Signal transduction
Specifications
Excitation Wavelength Range (Fluorometry)200 to 1000nm
Emission Wavelength Range (Fluorometry)360 to 820nm
Sensitivity (Fluorometry)Fluorescence intensity: <2 fmol fluorescein/well, 384-well plate; Time-resolved fluorescence: <20 amol Europium/well, 384-well plate
PrecisionFluorescence Polarization: <10mP

1nm Fluorescein, 96-well plate

Dynamic Range (Fluorometry)>5 decades
Wavelength Range (Luminometry)Standard mode: 360 to 820 nm; High-sensitive mode: 300 to 630nm
Sensitivity (Luminometry)High-sensitive mode: <10 amol ATP/well, 384-well plate; Standard mode: <200 amol ATP/well, 384-well plate
Dynamic Range (Luminometry)>5 decades
Wavelength Range (Photometry)200 to 1000nm
Measurement Range (Photometry)0 to 4.0 Abs
Linearity (Photometry)0 to 2.5 Abs (96-well plates) at 450nm, ±2%,

0 to 2 Abs (384-well plates) at 450nm, ±2%

No. of DispensersUp to two
Dispensing Volume5 to 500µL with 1µL increments
Incubator Temperature RangeFrom ambient +4°C to 45°C, at ambient 25°C, at ambient 25°C
ShakingLinear shaking
Plate Types6 to 384-well plates
Wavelength SelectionFilters (Ex/Abs 12.5mm, Em 25.4mm)
Measurement TypesFluorescence intensity, time-resolved fluorescence, fluorescence polarization, absorbance and luminescence
Light SourceXenon flash lamp
User InterfaceRequires, but does not include, a personal computer
Computer InterfaceSerial RS-232-C port
W x D x H14.8 x 19.5 x 13.5 in. (37.5 x 49.5 x 34cm)
Weight27kg (60 lbs.)

Cat. No.Description
Instruments
5230000Appliskan
5230010Appliskan with one dispenser
5230020Appliskan with two dispensers
Accessories
460SP400First Dispenser Kit
460SP420Second Dispenser Kit
 
 
 
 
 
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