Fluoroskan Ascent FL Microplate Fluorometer and Luminometer ­ Thermo Scientific

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Fluoroskan Ascent FL Microplate Fluorometer and Luminometer

Thermo Scientific Fluoroskan Ascent FL Microplate Fluorometer and Luminometer is ideal for life science research applications.
The Thermo Scientific Fluoroskan Ascent FL Microplate Reader? a compact and robust instrument with excellent optical performance for a variety of fluorometric and luminometric research applications, offering versatile plate formats, fast reading speeds, up to three dispensers and top/bottom reading of plates.
 
 
 
 
Part Number Description   Quantity
5210450 Fluoroskan Ascent FL 100-240V, 50/60Hz
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5210460 Fluoroskan Ascent FL 100 - 240V, 50/60Hz with one dispenser
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5210462 Fluoroskan Ascent FL 100-240V, 50/60Hz with two dispensers
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5210463 Fluoroskan Ascent FL 100-240V, 50/60Hz with three dispensers
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Please Note: Availability may vary by country.  
 
Fast reading speed

  • Reads a 96-well plate in just 15 seconds, which is essential for kinetic applications
  • Great for a wide variety of applications, such as cytotoxicity, ion channel, FRET and BRET
  • Combination of fluorometric GFP measurement and a lumometric luciferase measurement from the same well is fast due to the versatility of assay programming

Onboard dispensers for exact follow-up of kinetic reactions

  • Equipped with up to three reagent dispensers for�rapid kinetic assays, such as Ca2+ flux
  • Supports simultaneous dispensing and reading, enabling monitoring of kinetic measurements from the very start of the reaction
  • Very low dead volume and the backflush capability allow conservation of expensive reagent

High sensitivity for both top and bottom reading

  • Fiberless direct illumination optics for both top and bottom reading
  • High sensitivity, wide dynamic range and low cross talk ensure accurate and precise results for 1- to 384-well plates

Specially designed for automation

  • Reads up to 384-well plates for increased assay throughput
  • Easily integrated with automated systems
Includes: PC Software and filter pairs: Excitation: 355nm/Emission: 460nm, Excitation: 485nm/ Emission: 538nm

Warranty and Service Offering: 1 year

Recommended for: Ca2+ flux assays; Cell proliferation; Cytotoxicity; Cell adhesion; DNA quantitation; Reporter gene assays; Hybridization assays; Quantitation of PCR products; FRET assays; BRET assays; Molecular beacon assays; Immunoassays; Enzyme activity; Bacterial quantitation; Phagocytosis; Oligonucleotide assays
Specifications
Excitation Wavelength RangeExcitation: 320- 700nm; Emission: 360 - 670nm
Excitation FiltersUp to eight filters in the excitation filter wheel. 355 and 485nm filters included as standard. Other filters available upon request.
Emission FiltersUp to six filters in the emission filter wheel. 460 and 538nm filters included as standard. Other filters available upon request.
SensitivityFluorometry: 2 fmol fluorescein/well in a black 96-well plate

Luminometry: 40 amol ATP/well using flash reaction, white 384-plate

Dynamic RangeFluorometry: >6 decades

Luminometry: >9 decades over whole gain setting area

No of dispensersUp to 3
Dispensing volume1 to 1000µL in 1µL increments
Dispensing Speed25 seconds, 96-well plate, 5µL/well
Plate Types1- to 384-well plates
Measurement Speed15 seconds, 96-well plate
Wavelength selectionFilters
Light SourceQuartz-halogen Lamp
DetectorPhotomultiplier Tube
IncubatorFrom ambient +3°C to 45°C, at ambient 25°C
ShakingOrbital shaker
Computer InterfaceSerial RS-232C port
User InterfaceRequires, but does not include, a personal computer
Dimensions (HxWxD)13.4 x 16.5 x 16.5 in. (34 x 42 x 42cm)
WeightBasic unit: 21kg (46 lbs.); three optional dispensers add 3.5kg

Cat. No.No. of Dispensers
52104500
52104601
52104622
52104633
Includes PC Software and filter pairs: Ex 355nm/ Em 480nm, Ex 485nm/ Em 538nm, Other filters available upon request
 
 
 
 
 
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